文献类型：期刊文献

英文题名：Specific tracking of monoamine oxidase A in heart failure models by a far-red fluorescent probe with an ultra large Stokes shift

作者：Li, Xinming[1];Shi, Donglei[1];Song, Yihe[1];Xu, Yixiang[1];Gao, Ying[2];Qiu, Wenjing[1];Chen, Xin[1];Li, Xiaokang[1];Huang, Yunyuan[1];Feng, Yanjun[1];Li, Baoli[1];Guo, Yuan[2];Li, Jian[1,3,4,5]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Northwest Univ, Coll Chem & Mat Sci, Minist Educ, Key Lab Synthet & Nat Funct Mol, Xian 710127, Peoples R China;[3]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China;[4]Dali Univ, Coll Pharm & Chem, Dali 671000, Peoples R China;[5]Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Clin Med Sci & Tech Innovat Ctr, Shanghai 200092, Peoples R China

年份：2022

卷号：33

期号：3

起止页码：1572

外文期刊名：CHINESE CHEMICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000772416400080)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 220370 02, 220 07032 and 21977082), the National Mega-project for Innovative Drugs of China (No. 2019ZX09721001-004-003), the Chinese Postdoctoral Science Foundation, China (No. 2019M660083), the Shanghai Sailing Program, China (No. 20YF1411200).

语种：英文

外文关键词：Heart failure; Monoamine oxidase A; Oxidative stress; Fluorescent probe; Far-red emission; Diagnosis

摘要：Monoamine oxidase A (MAO-A) is a prominent myocardial source of reactive oxygen species (ROS), and its expression and activity are strongly increased in failing hearts. Therefore, accurate evaluation of MAO-A activity in cardiomyocytes is of great importance for understanding its biological functions and early diagnosing the progression of heart failure. However, so far, there is no report on the fluorescent diagnosis of heart failure by a specific probe for MAO-A. In this work, two far-red emissive fluorescent turn-on probes (KXS-M1 and KXS-M2) for the highly selective and sensitive detection of MAO-A were fabricated. Both probes exhibit good response to MAO-A, one of which, KXS-M2, performs better than the other one in terms of a fluorescence increment and sensitivity. Using the pioneering probe KXS-M2, specific fluorescence imaging of MAO-A in glucose-deprived H9c2 cardiac cells, zebrafish and isoprenaline-induced failing heart tissues was achieved, proving that KXS-M2 can serve as a powerful tool for the diagnosis and treatment of heart failure. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.