文献类型：期刊文献

中文题名：酰基激酶和酰基CoA合成酶对螺旋霉素合成的影响

英文题名：Influence of Acylkinase and Acyl-CoA Synthetase on the Biosynthes of Spiramycin by Streptomyces ambofaciens

作者：李友元[1];陈长华[1];李永东[1];李东红[1];陈国豪[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：27

期号：3

起止页码：251

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：螺旋霉素;酰基激酶;酰基CoA合成酶;影响因子;生物合成;激活作用;抗生素

外文关键词：spiramycin; acylkinase; acyl CoA synthetase; influencing factors

摘要：螺旋霉素链霉菌生物合成螺旋霉素的过程受许多酶的调控作用。酰基激酶和酰基 Co A合成酶是螺旋霉素内酯环合成的重要酶。实验测定了它们的酶活趋势和酶的影响因子。结果表明 ,酰基激酶和酰基 Co A合成酶都有两个活力峰 ,前者活性集中在发酵中前期 ,后者活性集中在发酵中后期。实验发现 Co2 + 、Mn2 + 对酰基激酶和酰基 Co A合成酶有较强的激活作用 ,Cu2 + 对酰基激酶有抑制作用 ,但对酰基 Co A合成酶有很强的激活作用。在发酵中期向摇瓶中补加二价阳离子比在发酵初期加入有更明显的激活作用 ,平均效价最高提高了 31 %
The biosynthes of spiramycin by Streptomyces ambofaciens was affected by the enzymatic system including acyl CoA synthetases and acylkinases. The activity and the influencing factors of those two enzymes have been studied. Both acylkinase and acyl CoA synthetase have two activity peaks. The former was only present in the prophase and the metaphase; the latter was synthesized during the metaphase and the anaphase. The results indicated that acylkinase and acyl CoA synthetase could be activated by Co 2+ and Mn 2+ while Cu 2+ could inhibit the former but activate the latter. Adding the influencing factors in the metaphase have a distinct activation than in the prophase. The maximum of improvement of average production was 31%.