文献类型：期刊文献

英文题名：Determination of L-glutamate using flow injection analysis with immobilized L-glutamate oxidase reactor.

作者：Li, Q[1];Ye, B;Zhang, S;Yu, J

机构：[1]Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai.

年份：1994

卷号：10

期号：4

外文期刊名：Chinese journal of biotechnology

收录：MEDLINE(收录号：7780026);;PubMed

语种：英文

摘要：L-Glutamate oxidase (GOD) and horseradish peroxidase (HRP) were covalently coupled on alkylamine pretreated controlled pore glass (CPG) by means of glutaraldehyde. The immobilized enzymes were packed into a teflon tube and used in flow injection analysis (FIA) system for L-glutamate determination. A good linearity range was obtained at 0.1-2.0 mM, and the coefficient of variation was 0.7% (n = 8). More than 80 samples were measured within an hour. The stability of the immobilized GOD reactor was good, retaining 50% of its initial activity after 4 months storage in buffer at 4 degrees C. When the concentration of L-glutamate remained lower than 2.5 mM, the determination of L-glutamate in this system was not affected by pH and temperature within the range of 6.0-8.0 and 20-35 degrees C, respectively. The system was applied to determine L-glutamate in broth samples during L-glutamate fermentation and good correlations were achieved between results obtained with the FIA system, L-glutamate oxidase kit and Warburg's method.