文献类型：期刊文献

英文题名：Magnesium modification of a calcium phosphate cement alters bone marrow stromal cell behavior via an integrin-mediated mechanism

作者：Zhang, Jing[1,2];Ma, Xiaoyu[2,3];Lin, Dan[2,3];Shi, Hengsong[1,2];Yuan, Yuan[1,2,3];Tang, Wei[1,3];Zhou, Huanjun[1,2];Guo, Han[3];Qian, Jiangchao[1,3];Liu, Changsheng[1,2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2015

卷号：53

起止页码：251

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20151700773369);WOS:【SCI-EXPANDED(收录号:WOS:000353929800024)】；

基金：The authors wish to express their gratitude to the financial supports from National Basic Research Program of China (973 Program, No. 2012CB933600), National Natural Science Foundation of China (No. 31330028, No. 31470924), the 111 Project (B14018). This study was also supported by Program for New Century Excellent Talents in University (No. NCET-11-0640).

语种：英文

外文关键词：Magnesium/calcium phosphate cements; Cell adhesion; Osteogenic differentiation; Fibronectin; Integrin

摘要：The chemical composition, structure and surface characteristics of biomaterials/scaffold can affect the adsorption of proteins, and this in turn influences the subsequent cellular response and tissue regeneration. With magnesium/calcium phosphate cements (MCPC) as model, the effects of magnesium (Mg) on the initial adhesion and osteogenic differentiation of bone marrow stromal cells (BMSCs) as well as the underlying mechanism were investigated. A series of MCPCs with different magnesium phosphate cement (MPC) content (0 similar to 20%) in calcium phosphate cement (CPC) were synthesized. MCPCs with moderate proportion of MPC (5% and 10%, referred to as 5MCPC and 10MCPC) were found to effectively modulate the orientation of the adsorbed fibronectin (Fn) to exhibit enhanced receptor binding affinity, and to up-regulate integrin alpha 5 beta 1 expression of BMSCs, especially for 5MCPC. As a result, the attachment, morphology, focal adhesion formation, actin filaments assembly and osteogenic differentiation of BMSCs on 5MCPC were strongly enhanced. Further in vivo experiments confirmed that 5MCPC induced promoted osteogenesis in comparison to at her CPC/MCPCs. Our results also suggested that the Mg on the underlying substrates but not the dissolved Mg ions was the main contributor to the above positive effects. Based on these results, it can be inferred that the specific interaction of Fn and integrin alpha 5 beta 1 had predominant effect on the MCPC-induced enhanced cellular response of BMSCs. These results provide a new strategy to regulate BMSCs adhesion and osteogenic differentiation by adjusting the Mg/Ca content and distribution in CPC, guiding the development of osteoinductive scaffolds for bone tissue regeneration. (C) 2015 Elsevier Ltd. All rights reserved.