文献类型：期刊文献

英文题名：A recombinant protein TmSm(T34A) can inhibit proliferation and proapoptosis to breast cancer stem cells(BCSCs) by down-regulating the expression of Cyclin D1

作者：Ma, Xingyuan[1,2,3,4];Zhang, Yi[1,2];Kang, Yanyan[1,2,3,4];Li, Linfeng[3];Zheng, Wenyun[4]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Clin Stem Cell Res Ctr, Shanghai 200127, Peoples R China;[4]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2016

卷号：84

起止页码：373

外文期刊名：BIOMEDICINE & PHARMACOTHERAPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390438100041)】；

基金：This research was supported by the Shanghai Pujiang Program (13PJD012), National Natural Science Foundation (30873190, 31300660), Science and technology innovation action plan of Shanghai (14431904300), and a foundation for young teacher from Education Ministry of China (20120074120027) and the National Science Research Project "Significant New Drugs Created" of Eleventh Five-year Plan (2009ZX09103-693), partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：TmSm(T34A); BCSCs; Proliferation; Proapoptosis; Cyclin D1

摘要：Cancer stem cells (CSCs), a small fraction of cancer cells lines proved with stem cell characteristics, were regarded as "bad seeds" related to recurrence, metastasis and chemotherapy resistance of breast carcinoma in recent years. So inhibiting the growth or inducing the differentiation and apoptosis of CSCs were considered as one of the effective pathways to fight against breast cancer. Based on the recombinant protein TmSm(T34A) that was designed and prepared in our previous experiments for targeting survivin, an inhibitor of apoptosis protein(IAP), in this study, we explored the effects of TmSm(T34A) on BCSCs obtained by enriching in serum-free suspension, sorting and characterizing of MCF-7/ADM. The results showed that TmSm(T34A) could not only inhibit the proliferation and growth of BCSCs by decreasing CD44(+)CD24(-) proportion and down-regulating the expression of Cyclin D1 significantly, but also induce BCSCs apoptosis evidently. Furthermore, in BCSCs xenograft nude mice administrated TmSm(T34A), the tumor growth was slower than that of the control obviously. Thus it can be seen TmSm(T34A) would be a promising potential protein for treatment of breast cancer by effecting on BCSCs. (C) 2016 Elsevier Masson SAS. All rights reserved.