文献类型：期刊文献

英文题名：Multilocus genotyping of Giardia duodenalis in Tibetan sheep and yaks in Qinghai, China

作者：Jin, Yue[1];Fei, Jilan[1];Cai, Jinzhong[2];Wang, Xiaolan[1];Li, Na[3];Guo, Yaqiong[3];Feng, Yaoyu[1,3];Xiao, Lihua[4]

机构：[1]East China Univ Sci & Technol, Sch Resource & Environm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qinghai Acad Vet Med & Anim Sci, Xining 810016, Qinghai, Peoples R China;[3]South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China;[4]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA

年份：2017

卷号：247

起止页码：70

外文期刊名：VETERINARY PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000415774800012)】；

基金：This work was supported by the National Natural Science Foundation of China (31425025, 31630078, 31602042), Water Special Project (2014ZX07104006).

语种：英文

外文关键词：Giardia duodenalis; Transmission; Tibetan sheep; Yaks; Subtyping

摘要：Giardia duodenalis is a common gastrointestinal protozoon in mammals. Although many studies have been reported on the distribution of G. duodenalis genotypes in sheep and cattle raised under intensive farming, few studies are available on the distribution of G. duodenalis in Tibetan sheep and yaks, which are raised free ranging in a continental plateau climate. In this study, 495 fecal specimens from Tibetan sheep and 605 from yaks were collected from eight counties in Qinghai, China and analyzed for G. duodenalis by PCR targeting the beta-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes. Based on PCR positivity at the bg locus, G. duodenalis occurrence rates were 13.1% (65/495) in Tibetan sheep and 10.4% (63/605) in yaks. DNA sequence analysis identified the presence of G. duodenalis Assemblages A (in 10 Tibetan sheep and 2 yaks) and E (in 51 Tibetan sheep and 60 yaks). In addition, mixed infections of the two were identified in four Tibetan sheep and one yak. Among the sequences obtained in this study, 1, 10, and 2 new subtypes of Assemblage E were detected at the bg, gdh and tpi loci, respectively. Based on sequences from the three loci, 28 multilocus genotypes (MLGs) were obtained, including 27 MLGs in Assemblage E and one MLG in Assemblage A. Each MLG was found in no more than seven animals, with most MLGs forming host-specific clusters in phylogenetic analysis except for one cluster including MLGs from both Tibetan sheep and yaks. Only two MLGs were found in both sheep and yaks. The above results demonstrate a high subtype diversity of G. duodenalis Assemblage E in Tibetan sheep and yaks raised in a traditional animal husbandry system and suggest that only limited cross-species transmission of G. duodenalis occurs between yaks and sheep sharing pastures.