文献类型：期刊文献

英文题名：Distinctive effect on nerve growth factor-induced PC12 cell neurite outgrowth by two unique neolignan enantiomers from Illicium merrillianum

作者：Tian, Xinhui[1];Yue, Rongcai[2];Zeng, Huawu[1];Li, Honglin[3];Shan, Lei[1];He, Weiwei[3];Shen, Yunheng[1];Zhang, Weidong[1,2]

机构：[1]Second Mil Med Univ, Sch Pharm, Dept Phytochem, Shanghai 200433, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2015

卷号：5

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000365018400002)】；

基金：The work was supported by the following programs: NSFC (81373301, 81230090, 1302658), Shanghai Leading Academic Discipline Project (B906), Shanghai Engineering Research Center for the Preparation of Bioactive Natural Products (10DZ2251300), Scientific Foundation of Shanghai, China (12401900801, 13401900101) and the National Key Technology R&D Program of China (2012BAI29B06).

语种：英文

摘要：Merrillianoid (1), a racemic neolignan possessing the characteristic benzo-2,7-dioxabicyclo[3.2.1] octane moiety, was isolated from the branches and leaves of Illicium merrillianum. Chiral separation of 1 gave two enantiomers (+)-1 and (-)-1. The structure of 1 was established by comprehensive spectroscopic analysis and single crystal X-ray diffraction. The absolute configurations of enantiomers were determined by quantum mechanical calculation. Compound (+)-1 exhibited a better neurotrophic activity than racemate 1 by promoting nerve growth factor (NGF) induced PC12 cell neurite outgrowth, while (-)-1 showed a distinctive inhibitory effect. Furthermore, a mechanism study indicated that the two enantiomers influenced NGF-induced neurite outgrowth of PC12 cells possibly by interacting with the trkA receptor, and extracellular signal regulated kinases 1/2 (ERK1/2) and mitogen-activated protein kinase (MEK) in Ras/ERK signal cascade. But the phosphorylation level of serine/threonine kinase Akt1 and Akt2 in PI3K/Akt signal pathway showed no significant difference between (+)-1 and (-)-1.