文献类型：期刊文献

英文题名：Recapitulation of growth factor-enriched microenvironment via BMP receptor activating hydrogel

作者：Zhang, Qinghao[1,2,3,4];Liu, Yuanda[1,2,3,4];Li, Jie[1,2,3,4];Wang, Jing[1,2,3,5];Liu, Changsheng[1]

机构：[1]East China Univ Sci & Technol, Mat Sci & Engn Sch, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Meilong Rd 130, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：20

起止页码：638

外文期刊名：BIOACTIVE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000830023100003)】；

基金：All surgical procedures were approved by the Institutional Animal Care and Use Committee of East China University of Science and Technology. This work was financially supported by the National Natural Science Foundation of China for Innovative Research Groups (Grant No. 51621002) , the Fundamental Research Funds for the Central Universities, State Administration of Foreign Experts Affairs P.R. China (Grant No. B14018) , National Natural Science Foundation of China (No. 31870953) ; National Key R & D Program of China (2018YFE0201500) . Qinghao Zhang and Yuanda Liu contributed equally to this work.

语种：英文

外文关键词：BMP-2; Sulfonated acids; BMP receptor Activation; Endogenous growth factor acquisition; Bone regeneration

摘要：Exposure to a growth factor abundant milieu has remarkable regenerative and rejuvenating effects on organ diseases, tissue damage, and regeneration, including skeletal system defects and bone regeneration. Although the introduction of candidate growth factors into relevant fields has been reported, their regenerative effects remain unsatisfactory, mainly because of the experimental challenges with limited types of growth factors, elusive dosage adjustment, and asynchronous stem cell activation with cytokine secretion. Here, an innovative hydrogel recapitulating a growth factor-enriched microenvironment (GEM) for regenerative advantage, is reported. This sulfated hydrogel includes bone morphogenetic protein-2 (BMP-2), an essential growth factor in osteogenesis, to direct mesenchymal stem cell (MSC) differentiation, stimulate cell proliferation, and improve bone formation. The semi-synthetic hydrogel, sulfonated gelatin (S-Gelatin), can amplify BMP-2 signaling in mouse MSCs by enhancing the binding between BMP-2 and BMP-2 type II receptors (BMPR2), which are located on MSC nuclei and activated by the hydrogel. Importantly, the dramatically improved cytokine secretion of MSCs throughout regeneration confirms the growth factor-acquiring potential of S-Gelatin/rhBMP-2 hydrogel, leading to the vascularization enhancement. These findings provide a new strategy to achieve an in situ GEM and accelerated bone regeneration by amplifying the regenerative capacity of rhBMP-2 and capturing endogenous growth factors.