文献类型：期刊文献

英文题名：In Situ Release of VEGF Enhances Osteogenesis in 3D Porous Scaffolds Engineered with Osterix-Modified Adipose-Derived Stem Cells

作者：Xu, Wan-Lin[1,2,3,4];Ong, Hui-Shan[1,2,3];Zhu, Yun[1,2,3];Liu, Sheng-Wen[1,2,3];Liu, Li-Min[1,2,3];Zhou, Kai-Hua[1,2,3];Xu, Zeng-Qi[4];Gao, Jun[5];Zhang, Yan[6];Ye, Jin-Hai[4];Yang, Wen-Jun[1,2,3]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Oral & Maxillofacial Head & Neck Oncol, Shanghai 200011, Peoples R China;[2]Shanghai Key Lab Stomatol, Shanghai, Peoples R China;[3]Shanghai Res Inst Stomatol, Shanghai, Peoples R China;[4]Nanjing Med Univ, Affiliated Hosp Stomatol, Dept Oral & Maxillofacial Surg, Jiangsu Key Lab Oral Dis, Nanjing 210029, Jiangsu, Peoples R China;[5]Nanjing Med Univ, Dept Neurobiol, Key Lab Human Funct Genom Jiangsu, Nanjing, Jiangsu, Peoples R China;[6]East China Univ Sci & Technol, Shanghai Key Lab Adv Polymer Mat, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai, Peoples R China

年份：2017

卷号：23

期号：9-10

起止页码：445

外文期刊名：TISSUE ENGINEERING PART A

收录：;EI(收录号：20172003683041);WOS:【SCI-EXPANDED(收录号:WOS:000401342400008)】；

基金：This study was supported by the National Natural Science Foundation of China (81371123 and 81302359), the Jiangsu Natural Science Foundation (BK2012844), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, 2014-37), and the Qing Lan Project through grants awarded to J.H-Ye. This work was also partially supported by the National Natural Science Foundations of China (No. 81222013) with a grant awarded to J Gao and the Research Grants (15411950300) from Science and Technology Commission of Shanghai Municipality.

语种：英文

外文关键词：Osterix; ADSCs; VEGF; PCL scaffolds; control release; osteogenesis

摘要：Adipose-derived stem cells (ADSCs) can differentiate into various cell types and thus have great potential for regenerative medicine. Herein, rat ADSCs were isolated; transduced with lentiviruses expressing Osterix (Osx), a transcriptional factor essential for osteogenesis. Osx overexpression upregulated key osteogenesis-related genes, such as special AT-rich binding protein 2, alkaline phosphatase, osteocalcin, and osteopontin, at both mRNA and protein levels. In addition, mineral nodule formation and alkaline phosphatase activity were enhanced in Osx-overexpressing ADSCs. The expression of dickkopf-related protein 1, a potent Wnt signaling pathway inhibitor, was also increased, whereas that of beta-catenin, an intracellular signal transducer in the Wnt pathway, was decreased. beta-catenin expression was partially recovered by treatment with lithium chloride, a canonical Wnt pathway activator. The Osx-expressing ADSCs were then combined with 3D gelatin-coated porous poly(e-caprolactone) scaffolds with a unique release prolife of entrapped recombinant human vascular endothelial growth factor (VEGF). The controlled release of VEGF promoted osteogenic differentiation capacity in vitro. When the scaffold-ADSC complexes were transplanted into rat calvarial critical-sized defects, more bone formed on the gelatin/VEGF-coated scaffolds than on other scaffold types. Taken together, the results indicate that, Osx-overexpression promotes ADSCs' osteogenesis both in vitro and in vivo, which could be enhanced by release of VEGF.