文献类型：期刊文献

英文题名：A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo

作者：Li, Yuyao[1];Song, Heng[1];Xue, Chenghong[1];Fang, Zhijun[1];Xiong, Liqin[2];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai Med X Engn Ctr Med Equipment & Technol, Shanghai 200030, Peoples R China

年份：2020

卷号：11

期号：23

起止页码：5889

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20202808912762);WOS:【SCI-EXPANDED(收录号:WOS:000544372300032)】；

基金：All animal procedures were performed in accordance with the Guidelines for the Care and Use of Laboratory Animals of Shanghai Jiao Tong University and approved by the Animal Ethics Committee of Shanghai Jiao Tong University. This study was financially supported by the Recruitment Program for Young Professionals (H. X.), the Research Program of State Key Laboratory of Bioreactor Engineering (H. X.), the Fundamental Research Funds for the Central Universities (H. X.), NSFC-BRICS (81861148020, H. X.), and the NSFC (81974273, L. X.).

语种：英文

外文关键词：Infrared devices - Quinone - Enzyme activity - Probes

摘要：Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic in vivo system.