文献类型：期刊文献

英文题名：Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source

作者：Zhang, Jian-Guo[1]; Wang, Xue-Dong[1]; Zhang, Ji-Ning[1]; Wei, Dong-Zhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, 200237, China

年份：2008

卷号：105

期号：4

起止页码：335

外文期刊名：Journal of Bioscience and Bioengineering

收录：EI(收录号：20082111269758)

语种：英文

外文关键词：Drug products - Fermentation - Metabolism - Organic carbon - Oxygen - Production control

摘要：In order to increase the yield of S-adenosylmethionine (SAM) in recombinant Pichia pastoris, a strategy of adding oxygen vectors and supplemental carbon sources was described. Three organic solutions were used as oxygen vectors for SAM accumulation at different concentrations and addition times. Firstly, n-hexane (0.5%) or n-heptane (1.0%) was added after 72 h of cultivation to improve SAM production. Carbon metabolism was scarce during the induction phase because of low methanol concentration. Secondly, sorbitol (1.2%), selected from three candidates (glycerol, lactic acid, and sorbitol), was used as the supplemental carbon source. The yield of SAM was improved significantly (53.26%) at 1.0% n-heptane added at 72 h (48 h induction), 1.2% sorbitol added at 72, 96, and 120 h of cultivation and 1.0% methanol added every 24 h during cultivation. ? 2008 The Society for Biotechnology, Japan.