文献类型：期刊文献

英文题名：Biomimetic Macroporous PCL Scaffolds for Ex Vivo Expansion of Cord Blood-Derived CD34+ Cells with Feeder Cells Support

作者：Pan, Xiuwei[1];Sun, Qiong[1];Zhang, Yuanhao[1];Cai, Haibo[1];Gao, Yun[1];Shen, Yongjia[1];Zhang, Weian[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai Key Lab Funct Mat Chem, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：17

期号：8

外文期刊名：MACROMOLECULAR BIOSCIENCE

收录：;EI(收录号：20172203706752);WOS:【SCI-EXPANDED(收录号:WOS:000409001400013)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21574039 and 21576087), the Science and Technology Commission of Shanghai Municipality (No. 15JC1401402), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：bioengineering; biomimetic; poly(epsilon-caprolactone); stem cells

摘要：Ex vivo expansion of hematopoietic stem cells (HSCs) with most current methods can hardly satisfy clinical application requirement. While in vivo, HSCs efficiently self-renew in niche where they interact with 3D extracellular matrix and stromal cells. Therefore, co-cultures of CD34(+) cells and mesenchyme stem cells derived from human amniotic membrane (hAMSCs) on the basis of biomimetic macro-porous three-dimensional (3D) poly(epsilon-caprolactone) (PCL) scaffolds are developed, where scaffolds and hAMSCs are applied to mimic structural and cellular microenvironment of HSCs. The influence of scaffolds, feeder cells, and contact manners on expansion and stemness maintenance of CD34(+) cells is investigated in this protocol. Biomimetic scaffolds-dependent co-cultures of CD34(+) cells and hAMSCs can effectively promote the expansion of CD34(+) cells; meanwhile, indirect contact is superior to direct contact. The combination of biomimetic scaffolds and hAMSCs represents a new strategy for achieving clinical-scale ex vivo expansion of CD34(+) cells.