文献类型：期刊文献

英文题名：Spatiotemporal Visualization of Cell Membrane with Amphiphilic Aggregation-Induced Emission Active Sensor

作者：Zhang, Youheng;Wang, Qi[1];Zhu, Zhirong;Zhao, Weijun;Yan, Chenxu;Liu, Zhenxing;Liu, Ming;Zhao, Xiaolei;Tian, He;Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Sch Chem & Mol Engn,Frontiers Sci Ctr Materiobiol, Joint Int Res Lab Precis Chem & Mol Engn,Inst Fin, Feringa Nobel Prize Scientist Joint Res Ctr,Shang, Shanghai 200237, Peoples R China

年份：2022

卷号：4

期号：5

起止页码：1619

外文期刊名：CCS CHEMISTRY

收录：WOS:【ESCI(收录号:WOS:000794318300017)】；

基金：This work was supported by the NSFC Science Center Program (no. 21788102), NSFC Major Research Project (no. 91959202), National Key Research and Development Program (no. 2016YFA0200300), NSFC/China (nos. 21974047 and 21622602), Pujiang Talents Plan (no. 19PJ1402300), China Postdoctoral Science Foundation (no. 2020M671328), Postdoctoral Science Foundation of Jiangsu Province (no. 2020Z189), Shanghai Municipal Science and Technology Major Project (no. 2018SHZDZX03), and Program of Introducing Talents of Discipline to Universities (no. B16017).

语种：英文

外文关键词：aggregation-induced emission; amphiphilicity; cell membrane imaging; high-fidelity; spatiotemporal dimensions

摘要：High-fidelity spatiotemporal monitoring of the cell membrane is critically important. However, commercial fluorescence probes are stalked by the aggregation-caused quenching (ACQ) effect, and the reported aggregation-induced emission (AIE)-active probes are always limited by nonspecific aggregations in the biological environment. Herein, we report the rational molecular design of a state-of-the-art amphiphilic AIE luminogen (AIEgen), membrane tracker QMC12, using a core quinoline-malononitrile (QM) structure to suppress the ACQ effect, incorporate a positively charged pyridinium to regulate dispersity and strengthen the binding affinity to the negatively charged cell membrane, and extend the alky chain to improve the anchoring ability to the cell membrane. The membrane tracker QMC12, which disperses well in both hydrophilic and lipophilic environments, not only achieves minimal background interference and high signal-to-noise (S/N) ratio in the "ultrafast" visualization of the cell membrane, but also endows a "wash-free" characteristic. Furthermore, it realizes a spatial three dimensional (3D) view in a multicellular spheroid model and morphology changes over time. Moreover, QMC12 avoids false staining and signal loss and unprecedentedly achieves the direct observation of the cell membrane's microstructure, which could elucidate spatiotemporal 3D model studies of the intercellular information exchange. [GRAPHICS] .