文献类型：期刊文献

英文题名：The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei

作者：Li, Ni[1];Qiu, Zhouyuan[1];Cai, Wanchuan[1];Shen, Yaling[1];Wei, Dongzhi[1];Chen, Yumeng[1];Wang, Wei[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 311,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Jiangsu Yiming Biol Technol Co Ltd, Suqian 223699, Jiangsu, Peoples R China

年份：2023

卷号：16

期号：1

外文期刊名：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

收录：;EI(收录号：20232214154117);WOS:【SCI-EXPANDED(收录号:WOS:000992797600001)】；

基金：This research was supported by the National Key Research and Development Program of China (2022YFA0912300), National Natural Science Foundation of China (32000050), Natural Science Foundation of Shanghai (No. 22ZR1417600), Chenguang Program of the Shanghai Education Development Foundation and Shanghai Municipal Education Commission (grant no. 21CGA34).

语种：英文

外文关键词：Ras small GTPase RSR1; Cyclic AMP; PKA; Filamentous fungi; Trichoderma reesei; Cellulase; Signaling

摘要：BackgroundLignocellulose is the most abundant renewable resource in the world and has attracted widespread attention. It can be hydrolyzed into sugars with the help of cellulases and hemicellulases that are secreted by filamentous fungi. Several studies have revealed that the Ras small GTPase superfamily regulates important cellular physiological processes, including synthesis of metabolites, sporulation, and cell growth and differentiation. However, it remains unknown how and to what extent Ras small GTPases participate in cellulase production.ResultsIn this study, we found that the putative Ras small GTPase RSR1 negatively regulated the expression of cellulases and xylanases. Deletion of rsr1 ( increment rsr1) significantly increased cellulase production and decreased the expression levels of ACY1-cAMP-protein kinase A (PKA) signaling pathway genes and the concentration of intracellular cyclic adenosine monophosphate (cAMP). Loss of acy1 based on increment rsr1 ( increment rsr1 increment acy1) could further increase cellulase production and the expression levels of cellulase genes, while overexpression of acy1 based on increment rsr1 ( increment rsr1-OEacy1) significantly reduced cellulase production and transcriptional levels of cellulase genes. In addition, our results revealed that RSR1 negatively controlled cellulase production via the ACY1-cAMP-PKA pathway. Transcriptome analysis revealed significantly increased expression of three G-protein coupled receptors (GPCRs; tre62462, tre58767, and tre53238) and approximately two-fold higher expression of ACE3 and XYR1, which transcriptionally activated cellulases with the loss of rsr1. increment rsr1 increment tre62462 exhibited a decrease in cellulase activity compared to increment rsr1, while that of increment rsr1 increment tre58767 and increment rsr1 increment tre53238 showed a remarkable improvement compared to increment rsr1. These findings revealed that GPCRs on the membrane may sense extracellular signals and transmit them to rsr1 and then to ACY1-cAMP-PKA, thereby negatively controlling the expression of the cellulase activators ACE3 and XYR1. These data indicate the crucial role of Ras small GTPases in regulating cellulase gene expression.ConclusionsHere, we demonstrate that some GPCRs and Ras small GTPases play key roles in the regulation of cellulase genes in Trichoderma reesei. Understanding the roles of these components in the regulation of cellulase gene transcription and the signaling processes in T. reesei can lay the groundwork for understanding and transforming other filamentous fungi.