文献类型：期刊文献

中文题名：水溶性亲和超滤载体的制备和应用

英文题名：Preparation of Dextran based Water Soluble Macroligand and Its Application

作者：朱家文[1];曹学君[2];武斌[1];戴干策[1];邬行彦[2]

机构：[1]华东理工大学化学工程研究所,上海200237;[2]华东理工大学生化工程系

年份：1999

卷号：25

期号：6

起止页码：563

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：2000045124489);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金

语种：中文

中文关键词：亲和超滤;葡聚糖;尿激酶;亲和载体;水溶性

外文关键词：affinity ultrafiltration; dextran T2000; urokinase; p aminobenzamidine; epichlorohydrin

摘要：用Ｄｅｘｔｒａｎ Ｔ２０００，经环氧氯丙烷交联并氧化产生部分羧基，偶联对氨基苯甲脒制得水溶性亲和载体巨配基。配基密度为７１．４μｍ ｏｌ／ｇ。截留分子量为１０ 万的超滤膜对所制亲和载体的截留率大于９９．５％ ，但尿激酶能自由通过。将比活为５００ＩＵ／Ａ２８０的尿激酶粗品经亲和超滤得到纯化的尿激酶，比活达６６ ３００ＩＵ／Ａ２８０，纯度提高１３３ 倍，回收率达８３．４％ 。
A water soluble urokinase specific macroligand was prepared by oxidizing and cross linking Dextran T2000 simultaneously with epichlorohydrin. The pendant carboxyl groups formed were then coupled with p aminobenzamidine. The ligand density of the macroligand was 71.4μmol/g dextran. A hollow fiber ultrafilter with 100 000 MWCO nominal (molecular weight cut off) membrane showed a rejection over 99.5% for the macroligand, but no retention for free urokinase by means of batchwise affinity ultrafitration the starting crude urokinase with specific activity of 500IU/ A 280 was purified with a yield of 83.4% and a purification factor of 132.