文献类型：期刊文献

英文题名：A novel signal amplification label based on AuPt alloy nanoparticles supported by high-active carbon for the electrochemical detection of circulating tumor DNA

作者：Chen, Kaicha[1];Zhao, Hongli[1];Wang, Zhenxing[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2021

卷号：1169

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20212010363161);WOS:【SCI-EXPANDED(收录号:WOS:000657650600003)】；

基金：This work was supported by the Natural Science Foundation of Shanghai (19ZR1412000) ; the Science and Technology Commission of Shanghai Municipality (STCSM, 20520712500) ; the Fundamental Research Funds for the Central Universities (JKJ01211718) ; and the Predict Project (Shanghai University No.18SKY00, AUT FFG No. 870027) . The authors thank the Research Center of Analysis and Test of East China University of Science and Technology for the help of Xray photoelectron spectrometer analysis.

语种：英文

外文关键词：Signal amplification; Bimetallic zeolitic imidazolate frameworks; AuPt alloy; Circulating tumor DNA; Sandwich-type electrochemical biosensor

摘要：The detection of circulating tumor DNA (ctDNA) has increasingly received a great deal of attention considering its significance in cancer diagnosis. And the signal amplification plays an important role in the development of sensitive ctDNA biosensors. Herein, the nanocomposites (denoted as HAC-AuPt), integrating from high-active carbon (HAC) and AuPt alloy nanoparticles, were synthesized and subsequently used as a signal amplification label to fabricate a sandwich-type ctDNA electrochemical biosensor. Characterizations demonstrated that HAC presents uniform size distribution and AuPt alloy nanoparticles were successfully loaded on HAC. The current response could be amplified to a great extent by the resultant HAC-AuPt due to its excellent electrochemical property. The nanocomposites were further bounded with DNA signal probes (SPs) via Au-S or Pt-S assembly to form SPs-label. After the capture probes (CPs) were immobilized on the electrode surface, the target DNA (tDNA) and SPs-label were stepwise incubated on the CPs-modified electrode, thus forming a sandwich-type structure. By monitoring the catalytic signal of HAC-AuPt towards the reduction process of H2O2, this biosensor provided a wide linear range of 10(-8) mol/L - 10(-16) mol/L with a low detection limit of 3.6 x 10(-17) mol/L (S/N = 3) for the detection of the tDNA. Furthermore, obvious differences in response signals among different DNAs were observed benefitting from the excellent selectivity of the biosensor. Besides, the long-term stability, reproducibility, and recovery rate were proved to be outstanding. These results indicate that the established biosensor holds a potential application in the clinical diagnosis of ctDNA. (C) 2021 Elsevier B.V. All rights reserved.