文献类型：期刊文献

英文题名：Highly Sensitive Ratiometric Self-Assembled Micellar Nanoprobe for Nitroxyl and Its Application In Vivo

作者：Yuan, Shaohua[1,2];Wang, Feiyi[1,2];Yang, Guichun[1,2];Lu, Cuifen[1,2];Nie, Junqi[1,2];Chen, Zuxing[1,2];Ren, Jun[1,2];Qiu, Yuan[3,4];Sun, Qi[3,4];Zhao, Chunchang[5,6];Zhu, Wei-Hong[5,6]

机构：[1]Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Hubei, Peoples R China;[2]Hubei Univ, Hubei Collaborat Innovat Ctr Adv Organ Chem Mat, Wuhan 430062, Hubei, Peoples R China;[3]Wuhan Inst Technol, Key Lab Green Chem Proc, Minist Educ, Wuhan 430205, Hubei, Peoples R China;[4]Wuhan Inst Technol, Sch Chem & Environm Engn, Wuhan 430205, Hubei, Peoples R China;[5]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[6]East China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2018

卷号：90

期号：6

起止页码：3914

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20181304938044);WOS:【SCI-EXPANDED(收录号:WOS:000428219600039)】；

基金：We gratefully acknowledge the financial support by the National Natural Science Foundation of China (21702053, 21676075), the Hubei Provincial Department of Education Project (Q20171013), and the Hubei Provincial Natural Science Foundation of China (2016CFB262, 2017CFB222).

语种：英文

外文关键词：Nitric oxide - Biocompatibility - Probes - Fluorescence

摘要：Nitroxyl (HNO) is a derivative of nitric oxide (NO) that plays an essential role in various biological and pharmacological events. Until now, the in situ trapping and specific detection of HNO in living samples is still challenging. In this project, we fabricated a novel BODIPY-based micellar nanoprobe for monitoring nitroxyl in vitro and in vivo in ratiometric mode in aqueous solution. The probe (P-BODIPY-N) contains an asymmetrical BODIPY dye for fluorescent signaling and a diphenylphosphinobenzoyl as the trigger moiety; then we encapsulated P-BODIPY-N into the hydrophobic interior of an amphiphilic copolymer (mPEG-DSPE) and prepared a novel BODIPY-based micellar nanoprobe: NP-BODIPY-N. As far as we know, this probe is the first reported ratiometric fluorescent nanoprobe for HNO, which exhibits ultrasensitivity, high selectivity, and good biocompatibility. Above all, this nanoprobe shows favorable cellular uptaken and was successfully used to detect intracellular HNO released by Angeli's salt in living cells and zebrafish larvae. These results indicate that our newly designed nanoprobe will provide a promising tool for the studies of HNO in living system.