文献类型：期刊文献

英文题名：Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products

作者：Cheng, Hui-Juan[1];Liu, Yue[1];Li, Hua-Dong[1,3];Yin, Bin-Cheng[1,2];Ma, Pei-Qiang[1];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Inst Engn Biol & Hlth, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China;[3]Chinese Acad Sci, Shanghai Adv Res Inst, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China

年份：2025

卷号：61

期号：34

起止页码：6300

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20251418185190);WOS:【SCI-EXPANDED(收录号:WOS:001457077200001)】；

基金：This work was jointly supported by the National Key Research and Development Program of China (Grant 2024YFA0917100), the National Natural Science Foundation of China (Grants 22104037, 22374047, 22134003) and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Biosensors

摘要：We combined catalytic hairpin assembly (CHA) with the Cas12a system for detecting melamine adulteration. This system involved two-step signal conversion and two-level amplification, boosting the sensor's versatility and sensitivity. The sensor showed excellent specificity and applicability for melamine detection in dairy products, and was broadened to viral nucleic acid detection.