文献类型：期刊文献

英文题名：Incorporating Lanthanum into Mesoporous Silica Foam Enhances Enzyme Immobilization and the Activity of Penicillin G Acylase Due to Lewis Acid-Base Interactions

作者：Tian, Chengcheng[1,2];Xu, Xuyang[1];Zhang, Haiyang[1];Mehio, Nada[3];Guo, Yanglong[4];Ma, Liang[1];Dai, Sheng[3,5]

机构：[1]East China Univ Sci & Technol, Natl Engn Lab Ind Wastewater Treatment, Shanghai 200237, Peoples R China;[2]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[3]Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA;[4]East China Univ Sci & Technol, Key Lab Adv Mat, Res Inst Ind Catalysis, Shanghai 200237, Peoples R China;[5]Oak Ridge Natl Lab, Div Chem Sci, Oak Ridge, TN 37831 USA

年份：2020

卷号：21

期号：15

起止页码：2143

外文期刊名：CHEMBIOCHEM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000527372700001)】；

基金：We are grateful to the National Natural Science Foundation of China (21103048, 21878099), the Shanghai Science and technology Innovation Plan (19DZ1208000), and 111 Project (B08021) for financial supports.

语种：英文

外文关键词：Immobilization; incorporation; La-MCF; Lewis acid; penicillin G acylase

摘要：Penicillin G acylase (PGA) has been immobilized on a lanthanum-incorporated mesostructured cellular foam (La-MCF) support by using the interaction between the strong Lewis acid sites on the surface of La-MCF and the free amino groups of lysine residues of PGA. The La-MCF support was successfully synthesized in situ through the addition of a citric acid (CA) complexant. The results of pyridine-IR spectroscopy show the presence of strong Lewis acid sites on the surface of the prepared La-MCF (with CA), attributed to the incorporation of lanthanum species into the framework of MCF. Through interaction with the strong Lewis acid sites, the enzymes can be firmly immobilized on the surface of the support. The results indicate that PGA/La-MCF (with CA) exhibits a high specific activity and greatly enhanced operational stability. For the hydrolysis of penicillin G potassium salt, the initial specific activity of PGA/La-MCF (with CA) reaches 10023 U/g. Even after being recycled 10 times, PGA/La-MCF (with CA) retains 89 % of its initial specific activity, much higher than the 77 % of PGA/Si-MCF.