文献类型：期刊文献

英文题名：Improved vitamin B₁₂ production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process

作者：Wang, Ze-Jian[1];Wang, Hui-Yuan[1];Li, Yong-Liang[2];Chu, Ju[1];Huang, Ming-Zhi[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Grp Hua Rong Pharmaceut Co Ltd, Shijiazhuang Pharma, Shijiazhuang 050041, Peoples R China

年份：2010

卷号：101

期号：8

起止页码：2845

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000274666100037)】；

基金：This work was financially supported by a Grant from the Major State Basic Research Development Program of China (973 Program), No. 2007CB774303, by Shanghai Leading Academic Discipline Project, No. 8505, and National Key Technology R&D Program, No. 2008BAl63B01. We also thanks Huarong pharmacy corporation (Shijiazhuang, China) for donating the industrial strain P. denitrificans.

语种：英文

外文关键词：Vitamin B-12; Oxygen uptake rate; Pseudomonas denitrificans; Scale-up; Oxygen supply strategy

摘要：Effects of different oxygen transfer rates (OTR) on the cell growth and vitamin B-12 biosynthesis of Pseudomonas denitrificans were first investigated under dissolved oxygen limiting conditions. The results demonstrated that high OTR accelerated cell growth and initial vitamin B-12 biosynthesis rate, while lower OTR was critical for higher productivity in the late fermentation process. The oxygen uptake rates (OUR) corresponded well with OTR. Based on the metabolic intermediate analysis, a step-wise OUR control strategy was proposed. The strategy was successfully implemented in scale-up to an industrial fermenter (120,000 A stable maximum vitamin B-12 production of 208 +/- 2.5 mg/l was achieved, which was increased by 17.3% compared with the control. Furthermore, the glucose consumption coefficient to vitamin B-12 was 34.4% lower than that of the control. An efficient and economical fermentation process based on OUR criterion was established for industrial vitamin B-12 fermentation by P. denitrificans. (C) 2009 Published by Elsevier Ltd.