文献类型：期刊文献

英文题名：de novo Fabrication of Dense Collagen Matrices with Patterned Hierarchical Structures for Corneal Stromal Tissue Repair

作者：Lei, Miao[1];Hou, Bing[1];Zhang, Shaohua[3,4,5];Chang, Ronghang[1];Ji, Yue[1];Chen, Kangli[1];Sun, Jianguo[3,4,5];Liu, Changsheng[1];Payne, Gregory F.[2];Qu, Xue[1,6,7]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai 200237, Peoples R China;[2]Robert E Fischell Biomed Device Inst, Inst Biosci & Biotechnol Res, 5118 A James Clark Hall, College Pk, MD 20742 USA;[3]Fudan Univ, Eye Inst, NHC Key Lab Myopia, Shanghai 200031, Peoples R China;[4]Fudan Univ, Dept Ophthalmol, NHC Key Lab Myopia, Shanghai 200031, Peoples R China;[5]Fudan Univ, Eye & ENT Hosp, Chinese Acad Med Sci, Key Lab Myopia,Shanghai Key Lab Visual Impairment, Shanghai 200031, Peoples R China;[6]East China Univ Sci & Technol, Sch Pharm, Shanghai Frontier Sci Res Base Optogenet Tech Cell, Shanghai 200237, Peoples R China;[7]Wenzhou Key Lab Tissue Regenerat Med Mat, Wenzhou 325000, Peoples R China

年份：2025

卷号：37

期号：39

外文期刊名：ADVANCED MATERIALS

收录：;EI(收录号：20252918799926);WOS:【SCI-EXPANDED(收录号:WOS:001527931600001)】；

基金：All the authors appreciate Mr. Yuan Zhuang, Ms. Huixian Gao and Ms. Mingyi Zou (Carl Zeiss Shanghai Co., Ltd) for their help with the confocal morphological observation tests, Dr. Xiaran Miu (BL19U2 beamline of Shanghai Synchrotron Radiation Facility) for help in synchrotron SAXS test, and All the authors thank the Research Center of Analysis and Test of East China University of Science and Technology for help on the material characterization and all the authors also appreciate the Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism for their valuable advices to this work. This work was jointly supported by the National Key Research and Development Program (2021YFB3800800), the National Natural Science Foundation of China (32425031, 32301113), the Science and Technology Innovation Project of Shanghai Science and Technology Committee (24CL2900800), the Zhejiang Natural Science Foundation (Z25E030005) and the Shanghai Sailing Program (23YF1409700). G.F.P. acknowledges the support from the U.S. National Science Foundation (CBET-1932963) and the Betty and Gordon Moore Foundation (11395).

语种：英文

外文关键词：collagen; de novo fabrication; dense organization; tailored mesoscale structures; patterning

摘要：Collagen is a versatile biological building block that is guided by extracellular cues to self-assemble into diverse hierarchical structures that perform various tissue functions. Here, the use of top-down inputs and controlled contextual cues is reported for the de novo fabrication of patterned dense collagen matrices with spatially varying tailored mesoscale structures. Initially, imposed electrical inputs are used to guide collagen triple helix molecules to partially assemble into a dense metastable molten fibril state that can be induced by permissive contextual cues (especially humidity) to undergo further assembly. Spatially-controlled optical inputs that induce riboflavin-based photo-crosslinking can locally-trap partially assembled states (e.g., molten fibrils). To illustrate the potential of collagen patterning, a functional corneal stromal substitute is specifically fabricated that mimics the microstructures of collagen in cornea-sclera and demonstrates that it can restore histological structure and function in a corneal defect rabbit model. This generic approach for the de novo fabrication of mesoscale-structured collagen is envisioned to have broad applications in regenerative medicine.