文献类型：期刊文献

英文题名：Dynamic Compartmentalization of Peptide-Oligonucleotide Conjugates with Reversible Nanovesicle-Microdroplet Phase Transition Behaviors

作者：Wang, Bin[1];Fang, Honglong[1];Zhu, Weiping[1];Xu, Yufang[1];Yang, Yangyang[1];Qian, Xuhong[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor, Shanghai 200237, Peoples R China

年份：2022

卷号：14

期号：32

起止页码：36998

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20223412597121);WOS:【SCI-EXPANDED(收录号:WOS:000837846700001)】；

基金：? ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China (Grant No. 31600802) , the Fundamental Research Funds for the Central Universities, and ?the Research Funds of Happiness Flower ECNU? (2020JK2103) .

语种：英文

外文关键词：liquid-liquid phase separation; nanovesicle; microdroplet; peptide-oligonucleotide conjugate; confinement effect

摘要：Developing artificial microsystems based on liquid-liquid phase separation (LLPS) to mimic cellular dynamic compartmentalization has gained increasing attention. However, limitations including complicated components and laborious fabrication techniques have hindered their development. Herein, we describe a new single-component dynamic compartmentalization system using peptide-oligonucleotide conjugates (POCs) produced from short elastin-like polypeptides (sELPs) and oligonucleotides (ONs), which can perform thermoreversible phase transition between a nanovesicle and a microdroplet. The phase transition of sELP-ONs is thoroughly investigated, of which the transition temperature can be controlled by concentration, length of sELPs and ONs, base sequences, and salt. Moreover, the sELP-ON microcompartment can enrich a variety of functional molecules including small molecules, polysaccharides, proteins, and nucleic acids. Two sELP-ON compartments are used as nano-and microreactors for enzymatic reactions, separately, in which chemical activities are successfully regulated under different-scaled confinement effects, demonstrating their broad potential application in matter exchange and artificial cells.