文献类型：期刊文献

英文题名：Synthesis of Abiotic Supramolecular Polymers Inside Living Cells via Organocatalysis-Mediated Self-Assembly

作者：Wang, Hucheng[1];Zheng, Ya-Ting[2,3];Zhang, Jiahao[1];Gao, Yuliang[1];Chen, Jingjing[1];Cai, Peiwen[1];Wang, Junyou[1];van Esch, Jan H.[4];Guo, Xuhong[1];Li, Hui[2,3,5];Wang, Yiming[1,6]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]Beijing Normal Univ, Sch Syst Sci, Beijing 100875, Peoples R China;[3]Beijing Normal Univ, Inst Nonequilibrium Syst, Beijing 100875, Peoples R China;[4]Delft Univ Technol, Dept Chem Engn, NL-2629 HZ Delft, Netherlands;[5]Beijing Normal Univ, Key Lab Cell Proliferat & Regulat Biol, Minist Educ, Beijing 100875, Peoples R China;[6]East China Univ Sci & Technol, Shanghai Key Lab Intelligent Sensing & Detect Tech, Shanghai 200237, Peoples R China

年份：2025

卷号：64

期号：21

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20251218088268);WOS:【SCI-EXPANDED(收录号:WOS:001445735500001)】；

基金：Y.W. acknowledges the financial support of the Natural Science Foundation of Shanghai (22ZR1417700), Shanghai Pilot Program for Basic Research (22TQ1400100-9), NSFC (21908061), the "Chenguang Program" supported by the Shanghai Education Development Foundation and Shanghai Municipal Education Commission (20CG36), and National Key Research and Development Program of China (2022YFD70050101). H.L. acknowledges the financial support from National Natural Science Foundation of China (12122402), and the Fundamental Research Funds for the Central Universities. The authors acknowledge Mr. Yunfei Xu from Hangzhou Surface Power Technology Co., Ltd. for the cytomechanics measurement using the Piuma Nanoindenter (Optics11 Life, Netherlands).

语种：英文

外文关键词：Gelation; Intracellular self-assembly; Local catalysis; Supramolecular polymers

摘要：Cells execute mesmerizing functions using supramolecular polymers (SPs) formed through the self-assembly of biological precursors. Integration of the vast array of synthetic SPs with living cells would offer a powerful way to remold cellular functions and bridge the gap between synthetic materials and the biological realm, yet remains a challenge because of the lack of robust abiotic SP systems that can be triggered to self-assemble inside cells. Here, we report how fully abiotic SPs can be synthesized inside living cells via an organocatalysis-responsive self-assembly strategy, and how the in situ-generated SPs are capable of interfering and can interfere with cellular functions. The incorporation of a nucleophilic organocatalyst (CAT) into living cells accelerates the intracellular conversion of hydrazide (H) and aldehyde-derived precursors (A) to hydrazone-based monomers (HA3) that locally self-assemble into SPs. Interestingly, the in situ-generated SPs possess ignorable effects on cell viability and proliferation but remarkably hinder cell migration. Furthermore, the presence of SPs is found to retard intracellular diffusion and alter the organization of the actin cytoskeleton, both of which are suggested to be responsible for the hindered cellular migration. In considering the vastly wide range of synthetic SPs, tremendous non-natural cellular functionalities can be obtained by in situ-synthesizing SPs.