文献类型：期刊文献

英文题名：Direct electrochemistry and electrocatalysis of hemoglobin immobilized on polyacrylamide-P123 film modified glassy carbon electrode

作者：Li, Jinxia;Tang, Jing;Zhou, Lihui[1];Han, Xia;Liu, Honglai

机构：[1]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2012

卷号：86

起止页码：60

外文期刊名：BIOELECTROCHEMISTRY

收录：;EI(收录号：20121814986169);WOS:【SCI-EXPANDED(收录号:WOS:000304638600008)】；

基金：This work was supported by the National Natural Science Foundation of China (Project No. 21176065), the Fundamental Research Funds for the Central Universities and the 111 Project of China (No. B08021).

语种：英文

外文关键词：Direct electron transfer; Hemoglobin; Polyacrylamide; P123

摘要：A novel organic mesoporous material, polyacrylamide-P123 (PAM-P123) composite film, was used to incorporate hemoglobin (Hb) onto the surface of glassy carbon (GC) electrode for studying the direct electron transfer of Hb and fabricating a sensitive biosensor of H2O2. Compared with inorganic mesoporous material, the PAM-P123 composite film has better film-forming property, which is particularly useful for preparing modified electrodes on various substrates for voltammetric measurements. The cyclic voltammetry of Nafion/Hb/PAM-P123/GC modified electrode showed a couple of well-defined and quasi-reversible redox peaks at about 0.317 V (vs. SCE) in pH 7.0 phosphate buffer solution. The Nafion/Hb/PAM-P123/GC modified electrode showed a fast amperometric response and good stability for the detection of hydrogen peroxide. The results indicate that the PAM-P123 film has a promising potential in fabricating third-generation electrochemical biosensors. (C) 2012 Elsevier B.V. All rights reserved.