文献类型：期刊文献

英文题名：Over-expression and characterization of recombinant prefoldin from hyperthermophilic archaeum Pyrococcus furiosus in E. coli

作者：Chen, Hui[2];Yang, Lida[1];Zhang, Yi[1,3];Yang, Shengli[1]

机构：[1]Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China;[2]E China Univ Sci & Technol, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Key Lab Synthet Biol, Shanghai 200233, Peoples R China

年份：2010

卷号：32

期号：3

起止页码：429

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20101012759022);WOS:【SCI-EXPANDED(收录号:WOS:000274542600015)】；

基金：This work is supported by National Basic Research Program of China (973 Program) No. 2004CB719606, 863 Program of China No. 2006AA02Z208, National Basic Research Program of China (973 Program) No. 2007CB714304, National Basic Research Program of China (973 Program) No. 2007CB707804 and the Key Project of CAS Knowledge Innovation Program KSC2-YW-G-005.

语种：英文

外文关键词：Escherichia coli; Prefoldin; Pyrococcus furiosus; Thermo-tolerance

摘要：Prefoldin is a hexameric chaperone that has been identified in eukaryotic cells and in Archaea. E. coli cells over-expressing the prefoldin gene from the hyperthermophilic, archaeum, Pyrococcus furiosus, grew well at 48A degrees C while control cells were unable to grow above 46A degrees C. The isolated and purified Pfu-prefoldin (specially the beta subunit and the prefoldin) thermally protected the activity of lysozyme.