文献类型：专利

英文题名：Method for detecting phenylalanine ketone in drug substance, involves preparing test solution by mixing phenylalanine ketone in water and reference solution by mixing phenylacetate in water followed by subjecting to chromatography

作者：PAN C;XIA W;XU Z;ZHANG W;FU D

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN104316641-B

公开日：2015-11-18

语种：英文

收录：DERWENT

摘要：NOVELTY - A phenylalanine ketone detection method involves preparing test solution by mixing known amount of phenylalanine ketone in water after sonication, where concentration of test solution is 1 mg.ml/l, preparing standard reference solution by mixing phenylacetate in water after sonication, where concentration of reference solution is 20 yg.ml/l followed by subjecting test solution and reference solution to chromatography under chromatographic conditions. USE - Method for detecting phenylalanine ketone in drug substance. ADVANTAGE - The method enables detecting phenylalanine ketone in drug substance with high sensitivity and better reproduction quality in an accurate manner. DETAILED DESCRIPTION - A phenylalanine ketone in drug substance detection method, involves preparing test solution by mixing known amount of phenylalanine ketone in water after sonication, where concentration of test solution is 1 mg.ml/l, preparing standard reference solution by mixing phenylacetate in water after sonication, where concentration of reference solution is 20 yg.ml/l followed by subjecting test solution and reference solution to chromatography under chromatographic conditions including octadecylsilane bonded silica column, mobile phase comprising 20 mmol/17 l phosphate having pH of 3.3, and potassium dihydrogen phosphate and acetonitrile in the volume ratio of 85:15, detection wavelength of 205 nm, column temperature of 30 degrees C and injection volume of 10 mu l.