文献类型：期刊文献

英文题名：De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

作者：Shi, Limin[1,2];Yan, Chenxu[1,2];Guo, Zhiqian[1,2];Chi, Weijie[3];Wei, Jingle[4];Liu, Weimin[4];Liu, Xiaogang[3];Tian, He[1,2];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Inst Fine Chem,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Joint Int Res Lab Precis Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Inst Fine Chem,Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Singapore Univ Technol & Design, Sci & Math Cluster, 8 Somapah Rd, Singapore 487372, Singapore;[4]ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China

年份：2020

卷号：11

期号：1

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000513504400020)】；

基金：This work was supported by NSFC/China (21788102, 21636002, 21622602, and 21908060), National Key Research and Development Program (2017YFC0906902 and 2016YFA0200300), Shanghai Municipal Science and Technology Major Project (Grant 2018SHZDZX03), the Innovation Program of Shanghai Municipal Education Commission, Scientific Committee of Shanghai (15XD1501400), Programme of Introducing Talents of Discipline to Universities (B16017), the Shuguang Program (18SG27), the China Postdoctoral Science Foundation (2019M651417), and Singapore University of Technology and Design (SUTD) and the SUTD-MIT International Design Centre (IDC) [T1SRCI17126, IDG31800104]. The authors would like to acknowledge the use of the computing service of SUTD-MIT IDC and National Supercomputing Centre, Singapore.

语种：英文

摘要：Fluorescence-based technologies have revolutionized in vivo monitoring of biomolecules. However, significant technical hurdles in both probe chemistry and complex cellular environments have limited the accuracy of quantifying these biomolecules. Herein, we report a generalizable engineering strategy for dual-emission anti-Kasha-active fluorophores, which combine an integrated fluorescein with chromene (IFC) building block with donor-pi-acceptor structural modification. These fluorophores exhibit an invariant near-infrared Kasha emission from the S-1 state, while their anti-Kasha emission from the S-2 state at around 520 nm can be finely regulated via a spirolactone open/closed switch. We introduce bio-recognition moieties to IFC structures, and demonstrate ratiometric quantification of cysteine and glutathione in living cells and animals, using the ratio (S-2/S-1) with the S-1 emission as a reliable internal reference signal. This de novo strategy of tuning anti-Kasha-active properties expands the in vivo ratiometric quantification toolbox for highly accurate analysis in both basic life science research and clinical applications.