文献类型：期刊文献

英文题名：Separation and purification of dl-tetrahydropalmatine from Corydalis yanhusuo by high-speed counter-current chromatography

作者：Liu, Zhilan[1]; Yu, Yan[1]; Shen, Pingniang[2]; Wang, Juan[2]; Wang, Chengyun[1]; Shen, Yongjia[1]

机构：[1] Laboratory for Advanced Materials, Institute of Fine Chemicals, East China University of Science and Technology, Shanghai, 200237, China; [2] National Engineering Research Center for Traditional Chinese Medicine, Shanghai, 201203, China

年份：2008

卷号：58

期号：3

起止页码：343

外文期刊名：Separation and Purification Technology

收录：EI(收录号：20074810945094)

语种：英文

外文关键词：Crude petroleum - High pressure liquid chromatography - Nuclear magnetic resonance spectroscopy - Optimization - Purification - Separation

摘要：High-speed counter-current chromatography (HSCCC) was applied to preparative separation and purification of dl-tetrahydropalmatine from Corydalis yanhusuo by a one-step separation. The two-phase system consisted of petroleum ether-ethyl acetate-methanol-water (15:30:21:20, v/v) was employed. An orthogonal design was used for optimizing the revolution speed of the separation column, flow rate of the mobile phase and separation temperature, which was 850 rpm, 1.2 ml/min and 20 °C, respectively. HPLC analysis of the fractions collected by preparative HSCCC of 200 mg of crude extracts showed that the purity of dl-tetrahydropalmatine (8.6 mg) was 96.4%. The chemical identity of the component was confirmed by 1H NMR and EI-MS. ? 2007 Elsevier B.V. All rights reserved.