文献类型：期刊文献

英文题名：A Novel Peptide-Equipped Exosomes Platform for Delivery of Antisense Oligonucleotides

作者：Xu, Huiying[1];Liao, Chong[1];Liang, Shifu[2];Ye, Bang-Ce[1,2,3]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Inst Engn Biol & Hlth, Hangzhou 310014, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832000, Xinjiang, Peoples R China

年份：2021

卷号：13

期号：9

起止页码：10760

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20211110085618);WOS:【SCI-EXPANDED(收录号:WOS:000629054100009)】；

基金：This work was supported by the National Natural Science Foundation of China (21705047), the National Key Research and Development Program of China (2020YFA0908800), and the Fundamental Research Funds for the Central Universities (222201814030).

语种：英文

外文关键词：exosomes; cell-penetrating peptides; antisense oligonucleotides; cellular delivery; gene silencing

摘要：Exosomes are natural delivery vehicles because of their original feature such as low immunogenicity, excellent biocompatibility, and migration capability. Engineering exosomes with appropriate ligands are effective approaches to improve the low cellular uptake efficiency of exosomes. However, current strategies face considerable challenges due to the tedious and labor-intensive operational process. Here, we designed a novel peptides-equipped exosomes platform which can be assembled under convenient and mild reaction condition. Cell-penetrating peptides (CPPs) was conjugated on HepG2 cells-derived exosomes surface which can not only enhance the penetrating capacity of exosomes but also assist exosomes in loading antisense oligonucleotides (ASOs). The cellular uptake mechanism was investigated and we compared the difference between natural exosomes and modified exosomes. The resulting nanosystem demonstrated a preferential tropism for cells that are parented to their source tumor cells and could remarkably increase the cellular delivery of G3139 with efficient downregulation of antiapoptotic Bcl-2. This work developed a rapid strategy for intracellular delivery of nucleic acids, thus providing more possibilities toward personalized cancer medicine.