文献类型：期刊文献

英文题名：Kinetically Controlled Synthesis of Cefaclor with Immobilized Penicillin Acylase in the Presence of Organic Cosolvents

作者：Zhang, Ye-Wang[1,2,4,5];Liu, Shu-Lai[1,3];Wei, Dong-Zhi[1];Liu, Rui-Jiang[2,4,5];Xu, Xi-Ming[2]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China;[3]Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310014, Zhejiang, Peoples R China;[4]Jiangsu Univ, United Res Ctr, Zhenjiang 212013, Zhenjiang, Peoples R China;[5]Sopo Xingda Pharmaceut Co Ltd, Zhenjiang 212013, Zhenjiang, Peoples R China

年份：2008

卷号：22

期号：3

起止页码：349

外文期刊名：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY

收录：;EI(收录号：20084211640264);WOS:【SCI-EXPANDED(收录号:WOS:000260089500010)】；

语种：英文

外文关键词：3-chloro-7-D-(2-phenylglycinamide)-3-cephem-4-carboxylic acid; organic cosolvents; penicillin acylase; enzymatic synthesis

摘要：Enzymatic syntheses of cefaclor with immobilized penicillin acylase in organic cosolvents under kinetic control were carried out. KcPGA from Kluyvera citrophila was selected as the best catalyst among the three species of immobilized penicillin acylase. Ethylene glycol, glycerol, methanol, ethyl acetate and polyethyleneglycol (PEG) were selected accordingly and cefaclor syntheses were preformed respectively. Best results in terms of yield were obtained in ethylene glycol, with which further studies were investigated and the maximum yield was Y = 93.5 %. The optimal conditions were pH 6.5, temperature theta = 5 degrees C, 3 mol D-phenylglycine methyl ester (PGME) per mol 7-aminodesacetoxymethyl-3-chlorocephalosporin acid (7ACCA) and x = 30 % ethylene glycol fraction. Under above mentioned conditions, the yield was Y = 91.1 %.