文献类型：期刊文献

中文题名：Pseudomonas aeruginosa W_3产鼠李糖脂的结构表征及理化性质

英文题名：Structural and physicochemical characterization of rhamnolipids produced by Pseudomonas aeruginosa W_3

作者：崔长征[1];沈萍[2];张甲耀[3];冯耀宇[1]

机构：[1]华东理工大学资源与环境工程学院国家环境保护化工过程环境风险评价与控制重点实验室,上海200237;[2]武汉大学生命科学学院,武汉430072;[3]武汉大学资源与环境科学学院,武汉430072

年份：2010

卷号：30

期号：10

起止页码：2030

中文期刊名：环境科学学报

外文期刊名：Acta Scientiae Circumstantiae

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家博士后特别资助基金项目(No. 200902093)~~

语种：中文

中文关键词：生物表面活性剂;鼠李糖脂;双鼠李糖脂;Pseudomonas;aeruginsa

外文关键词：biosurfactant; rhamnolipid; di-rhamnolipid; Pseudomonas aeruginosa

摘要：利用液相色谱/质谱联用仪(HPLC-ESI-MS)分析了石油降解菌Pseudomonas aeruginosa W3以甘露醇为碳源所产鼠李糖脂生物表面活性剂的组成.结果表明,所产鼠李糖脂共检出6种主要的鼠李糖脂同系物,均由1～2个鼠李糖分子和1～2个含β羟基的碳链长度为8～12的饱和或不饱和脂肪酸分子构成,其主要组分的m/z为649.6和621.5,对应的结构是RhaRhaC10C10和RhaRhaC8C10,分别占总检出物质量的57%和15.5%.该鼠李糖脂混合物中双鼠李糖脂的含量达到90%,是目前报道的双鼠李糖脂含量较高的菌株之一.该糖脂类生物表面活性剂可将水的表面张力从71.4mN.m-1降到30.5mN.m-1,临界胶束浓度为48mg·L-1,在高温、高盐度及高pH等极端环境下,仍能保持较高的表面活性和乳化能力,在生物修复中具有潜在应用价值.
The composition of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa W3 using mannitol as a carbon source was analyzed by liquid chromatography/mass spectrometry. Six rhamnolipid homologs were identified,with structures consisting of one or two rhamnose molecules linked to one or two β-hydroxy fatty acids with saturated or unsaturated alkyl chains between C8 and C12. The two major components are RhaRhaC10C10 （57%） and RhaRhaC8C10 （15.5%）,with m/z of 649.6 and 621.5,respectively. The concentration of di-rhamnolipid in the product can reach as high as 90%,one of the highest ratios in the literature. The rhamnolipid product can reduce the surface tension of water from 71.4 mN·m-1 to 30.5 mN·m-1,with a critical micelle concentration （CMC） of 48 mg·L-1. It also demonstrated high emulsifying activity and good thermal,pH and salt stability,exhibiting potential for bioremediation.