文献类型：期刊文献

英文题名：Metabolic engineering of erythritol production from glycerol by Yarrowia lipolytica

作者：Wang, Ya-Ting[1,2,3];Zhao, Ling-Xuan[1,2,3];Wei, Liu-Jing[1,2,3];Chen, Jun[1,2,3];Liu, Zhijie[1,2,3];Liu, Feng[1,2,3];Hua, Qiang[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hubei Univ Technol, Hubei Key Lab Ind Microbiol, Cooperat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn,Minist Educ,Minist Educ & H, Wuhan 430068, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2024

卷号：29

期号：1

起止页码：157

外文期刊名：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

收录：;EI(收录号：20240815568996);WOS:【SCI-EXPANDED(收录号:WOS:001163214800003)】；

基金：This study was supported by the National Natural Science Fund for Young Scholars (32001035), the National Key R&D Program of China (2017YFE0115600, 2019YFA0904302) and the 111 Project (B18022).

语种：英文

外文关键词：Erythritol; By-products; Yarrowia lipolytica; Metabolic engineering

摘要：Erythritol as a four-carbon polyol has been widely used in food, pharmaceutical and daily chemical industries with characteristics of low caloric value and high chemical stability. Here, a system metabolic engineering strategy was used to increase the yield of erythritol from glycerol in Yarrowia lipolytica by enhancing the substrate transformation and restricting the by-product synthesis. Specifically, we determined that over-expression of a newly identified erythrose reductase YPR1 was able to improve the erythritol production as same as the well-known erythrose reductase ER27. Instead of its up-regulation, knockout of erythrose reductase ER10 was effective to improve erythritol synthesis. Moreover, both over-expression of YPR1 and deletion of ER10 significantly accelerated the glycerol utilization in response to high osmotic stress. To further decrease the by-product accumulation, a restriction and recycling strategy was implemented by knockout of mannitol dehydrogenase MDH2 and enhancement of arabitol dehydrogenase ADH1 and fructokinase HXK1. The engineered strain YL13 produced a titer of 25 g/L erythritol and less than 0.5 g/L mannitol and arabitol. By over-expression of transketolase TKL1, the final strain YL14 produced 28.5 g/L erythritol and none of mannitol and arabitol. This study provides a new idea for reducing the production of by-products and improving the glycerol conversion to erythritol.