文献类型：期刊文献

英文题名：Strategy based on multiplexed brush architectures for regulating the spatiotemporal immobilization of biomolecules

作者：Zhao, Haili[1]; Chen, Tao[1]; Wu, Tong[3]; Xie, Linsheng[2]; Ma, Yulu[2]; Sha, Jin[2]

机构：[1] Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, 650504, China; [2] School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai, 200237, China; [3] Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China

年份：2022

卷号：141

外文期刊名：Biomaterials Advances

收录：EI(收录号：20224012834404)

语种：英文

外文关键词：Acrylic monomers - Cell adhesion - Dendrimers - Proteins

摘要：Functional surfaces that enable both spatial and temporal control of biomolecules immobilization have attracted enormous attention for various fields including smart biointerface materials, high-throughput bioarrays, and fundamental research in the biosciences. Here, a flexible and promising method was presented for regulating the spatiotemporal arrangement of multiple biomolecules by constructing the topographically and chemically diverse polymer brushes patterned surfaces. A series of polymer brushes patterned surfaces, including antifouling brushes patterned surface, epoxy-presenting brushes patterned surface without and with antifouling background layer, were fabricated to control the spatial distribution of protein and cell adhesion through specific and nonspecific means. The fluorescence measurements demonstrated the effectiveness of spatially regulating the density of surface-immobilized protein through controlling the areal thickness of the poly (glycidyl methacrylate) (PGMA) brush patterns, leading to various complex patterns featuring well-defined biomolecule concentration gradients. Furthermore, a multiplexed surface bearing epoxy groups and azido groups with various areal densities was fabricated for regulating the spatiotemporal arrangement of different proteins, enabling binary biomolecules patterns with higher degrees of functionality and complexity. The presented strategy for the spatiotemporal control of biomolecules immobilization would boost the development of dynamic and multifunctional biosystems. ? 2022 Elsevier B.V.