文献类型：期刊文献

英文题名：Monodisperse core-shell silica particles as a high-performance liquid chromatography packing material: Facile in situ silica sol-gel synthesis

作者：Wu, Yaling[1];Sun, Xiaotong[1];Zhu, Junchen[1];Shen, Jiawei[1];Wang, Huiying[1];Zhu, Lunan[1];Zhou, Yang[1];Ke, Yanxiong[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：1625

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20202408812615);WOS:【SCI-EXPANDED(收录号:WOS:000552017600027)】；

语种：英文

外文关键词：Core-shell silica particles; In situ silica sol-gel method; Poly(diallyldimethylammonium chloride); High-performance liquid chromatography

摘要：In recent years, core-shell silica particles (CSSPs) have been increasingly used for highly efficient separation at fast flow rates and relatively low back pressures in high-performance liquid chromatography (HPLC). However, material synthesis techniques for producing CSSPs economically in batch processes remain elusive. In this report, a practical and straightforward method for the preparation of CSSPs is presented. By refluxing freshly prepared nonporous silica particles in ammonia-water solution in the presence of poly(diallyldimethylammonium chloride) at 70-100 degrees C, CSSPs with shell thicknesses of up to 300 nm and pore sizes from 8 to 25 nm were easily prepared. The effects of the synthetic conditions on the shell thickness, surface area, and pore size were investigated in detail, and the method reproducibility was evaluated in scale-up experiments. A mechanism of CSSP formation is also proposed. The CSSPs were characterized via scanning electron microscopy, transmission electron microscopy, laser particle size (dynamic light scattering) analysis, and nitrogen adsorption and desorption experiments. The synthesized 3.4-mu m CSSPs were functionalized with dimethyloctadecylchlorosilane and used as an HPLC packing material, exhibiting excellent separation performance for both small molecules and large biomolecules. In summary, we report the simplest method developed thus far for the preparation of monodisperse core-shell silica particles suitable for HPLC column packing. (C) 2020 Elsevier B.V. All rights reserved.