文献类型：期刊文献

英文题名：In vitro and in vivo antioxidant activities of a flavonoid isolated from celery (Apium graveolens L. var. dulce)

作者：Li, Peng[1];Jia, Jia[1];Zhang, Daihui[1];Xie, Jingli[1];Xu, Xueshu[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, Dept Food Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：5

期号：1

起止页码：50

外文期刊名：FOOD & FUNCTION

收录：;EI(收录号：20140117150494);WOS:【SCI-EXPANDED(收录号:WOS:000328887900006)】；

基金：This work was supported by "the Fundamental Research Funds for the Central Universities", and "National Major Science and Technology Projects of China (no. 2012ZX09304009)".

语种：英文

外文关键词：Assays - Brain - Plants (botany) - Free radicals - Column chromatography - Antioxidants

摘要：In the present article, a flavonoid was separated and purified from celery leaf through ethanol extraction, column chromatography and crystallization. The product was identified as apiin by LC/ESI-MS, and its antioxidant activities were evaluated in vitro, including by 1,1-diphenl-2-picrylhyrazyl free radical (DPPH center dot), superoxide radical (O-2(-center dot)) and hydroxyl radical (OH center dot) scavenging assays. IC50 values were 68.0 mu g ml(-1) in the DPPH assay, 0.39 mg ml(-1) in the O-2(-center dot) assay and 48.0 mu g ml(-1) in the OH center dot assay. The antioxidant activities were investigated in vivo with the use of mice models. All data were measured including the contents of maleic dialdehyde (MDA) and lipofuscin (LPF), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and the total antioxidant capacity (TAOC), in the serum, brain, heart, liver and kidney. Results showed that apiin had a remarkable scavenging activity on MDA and LPF, promoted TAOC and significantly enhanced the activities of SOD, GSH-Px and CAT.