文献类型：期刊文献

中文题名：基于MALDI-TOF MS的串联富集策略在磷酸化蛋白组学中的应用

英文题名：Application of Tandem Enrichment Strategy by MALDI-TOF MS in Phosphorylated Proteomics

作者：张传静[1];吴婷[1];任红鑫[1];张玲帆[1];杜一平[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2017

卷号：36

期号：9

起止页码：1061

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家质检总局科技计划项目(2015IK201)

语种：中文

中文关键词：脱盐;固定金属离子亲和色谱;基质辅助激光解吸/电离飞行时间质谱;磷酸肽;串联富集

外文关键词：desalting ; IMAC ; matrix-assisted laser desorption - ionization time of flight mass spec-trometry（ MALDI - TOF MS） ; phosphopeptides; tandem enrichment

摘要：提出一种除盐-富集串联用于磷酸肽富集研究的思路。选用C18柱和铈(Ⅳ)修饰的壳聚糖材料进行脱盐实验,以制备的基于聚合物基体螯合Fe3+的亲和色谱材料为富集材料。将直接富集和串联策略应用到标准品和血清中,研究结果表明,该富集材料具有高选择性和高灵敏度(1.6 fmol),铈(Ⅳ)修饰的壳聚糖材料前提下的串联策略能明显降低样品的复杂性。相比直接富集方法,能够提高磷酸化肽的覆盖率。
A scheme was presented for the tandem purification of phosphopeptide using desalting prior to immobilized metal ion affinity chromatography（IMAC）.Both C18 tip and the cerium（Ⅳ）-modified chitosan material（Ce-MCM） were used in the desalting experiments.GMA-co-SR454/Iron（Ⅲ）-IDA was synthesized,and used as an excellent phosphopetides enrichment material.They were applied to selectively capture the phosphopeptides in standards and human serum.Results demonstrated that C18/IMAC and Ce-MCM/IMAC have different performances because of their hydrophobic or hydrophilic properties.Ce-MCM/IMAC tandem strategy notably decreased the complexity of peptides,and yielded a far greater coverage for phosphopeptides than single IMAC.