文献类型：期刊文献

英文题名：A novel "donor-two-acceptor" type fluorophore-based probe for fast detection and intracellular imaging of nitroreductase

作者：Li, Dandan[1];Xu, Yuqiong[1];Zhou, Nannan[2];Liu, Jianxu[2];Wang, Rui[2];Cheng, Tao[2];Tang, Yun[2];Zhu, Weiping[1];Xu, Yufang[1];Qian, Xuhong[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2017

卷号：136

起止页码：627

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20163802823919);WOS:【SCI-EXPANDED(收录号:WOS:000387837200075)】；

基金：This work is financially supported by the National Natural Science Foundation of China (Grants 21236002).

语种：英文

外文关键词：Probes - Solutions

摘要：By fusion of HBT and hemicyanine, we have designed a novel donor-two-acceptor type fluorophore (HHC) as a platform for the detection of different kinds of guests. HHC displays an emission at 580 nm in aqueous solution and two emission bands at 450 nm and 616 nm as a result of the donor-two-acceptor feature with the existance of CTAB. The fluorescence of HHC may be readily modulated by protection/deprotection of its electron-donating hydroxyl group and response different guests. As an example, we introduced p-nitrobenzyl group, which is sensitive to Nitroreductase (NTR), to the hydroxyl group of HHC and constructed a novel selective and sensitive fluorescence probe HHCP for fast detection and intracellular imaging of nitroreductase. (C) 2016 Elsevier Ltd. All rights reserved.