文献类型：期刊文献

英文题名：Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside

作者：Wei, Xiao-Kun[2];Ding, Qing-Bao[1];Zhang, Lu[3];Guo, Yong-li[1];Ou, Lin[1];Wang, Chang-lu[2]

机构：[1]E China Univ Chem Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China;[3]Shanghai Weiping Biosci & Biotechnol Co Ltd, Shanghai 200237, Peoples R China

年份：2008

卷号：9

期号：7

起止页码：520

外文期刊名：JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000257814100002)】；

语种：英文

外文关键词：nucleoside phosphorylase (NPase); Enterobacter aerogenes; cytidine; cytidine 5'-monophosphate (CMP); adenine arabinoside

摘要：Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5'-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase ( PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.