文献类型：期刊文献

英文题名：Colorimetric and Plasmonic Detection of Lectins Using Core-Shell Gold Glyconanoparticles Prepared by Copper-Free Click Chemistry

作者：Hu, Xi-Le[1,2];Jin, Hong-Ying[1,2];He, Xiao-Peng[1,2];James, Tony D.[3];Chen, Guo-Rong[1,2];Long, Yi-Tao[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

年份：2015

卷号：7

期号：3

起止页码：1874

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20150500476776);WOS:【SCI-EXPANDED(收录号:WOS:000348688700060)】；

基金：We thank the 973 project (2013CB733700), the National Natural Science Foundation of China (21176076, 21202045), the Key Project of Shanghai Science and Technology Commission (13NM1400900), and the Fundamental Research Funds for the Central Universities. The Catalysis And Sensing for our Environment (CASE) network is thanked for research exchange opportunities.

语种：英文

外文关键词：sugar; lectin; Au-NP; click chemistry; SPAAC; plasmon resonance scattering spectroscopy

摘要：This study describes the simple preparation of core-shell glycosyl gold nanoparticles (AuNPs) using stepwise, copper-free click chemistry-promoted self-assembly. The as-formed glyco-AuNPs can be used for the selective detection of sugar-lectin interactions, which are vital to many important physiological and pathological processes. The approach uses AuNPs as bioprobes since they produce, sensitively, changes in both color visible to the naked eye and surface plasmon resonance (SPR), on aggregation. Strain-promoted click reaction of an azido galactoside with a lipid cyclooctyne affords a galactolipid that can be embedded into polyethylene glycol (PEG)-coated AuNP via self-assembly. Subsequently, using naked-eye and plasmon resonance scattering spectroscopy, we were able to observe the colorimetric and plasmonic variations of the glyco-AuNPs, respectively, in the presence of a selective lectin over other proteins.