文献类型：期刊文献

英文题名：Enantioseparation of basic drugs by reverse phase high-performance liquid chromatography system using carboxymethyl-β-cyclodextrin as chiral mobile phase additive

作者：Ao, Qiong[1];Zhao, Hongliang[1];Tong, Tianzhong[1];Peng, Yangfeng[1];He, Zhaoyang[1]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：34

期号：8

起止页码：1128

外文期刊名：CHIRALITY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000795834400001)】；

语种：英文

外文关键词：basic drug; carboxymethyl-beta-cyclodextrin; chiral mobile phase additive; enantioseparation; HPLC

摘要：A rapid and efficient method was developed for enantioseparation of basic drugs, using carboxymethyl-beta-cyclodextrin (CM-beta-CD) as chiral mobile phase additive, rather than involving costly chiral column in high-performance liquid chromatography (HPLC) system. Four of the six basic drug enantiomers investigated were successfully separated. The highest resolution reaches 2.15 for threo-(1S,2S)-2-amino-l-p-nitrophenyl-1,3-propanediol. The effects of the organic modifier, pH value, concentration of chiral additive, column temperature, and flow rate of mobile phase on the enantioseparation of analytes were researched. The apparent formation constants of inclusion and the thermodynamic parameters were evaluated to explain the mechanism of chiral recognition.