文献类型：期刊文献

英文题名：Degradation controllable biomaterials constructed from lysozyme-loaded Ca-alginate microparticle/chitosan composites

作者：Wei, Lan[1];Cai, Chunhua[1];Lin, Jiaping[1];Wang, Liquan[1];Zhang, Xiangman[2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Adv Polymer Mat, Key Lab Ultrafine Mat,Sch Mat Sci & Engn, Minist Educ,State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Fudan Univ, Zhongshan Hosp, Dept Vasc Surg, Shanghai 200032, Peoples R China

年份：2011

卷号：52

期号：22

起止页码：5139

外文期刊名：POLYMER

收录：;EI(收录号：20114114424065);WOS:【SCI-EXPANDED(收录号:WOS:000296109300017)】；

基金：This work was supported by National Natural Science Foundation of China (50925308). Supports from projects of Shanghai Municipality (09XD1401400, 0952nm05100, 08DZ2230500, and B502) are also appreciated.

语种：英文

外文关键词：Alginate; Chitosan; Degradation

摘要：A degradation controllable composite material was designed to resist the degradation rate variation caused by pH change. The degradation controllable composite materials were prepared by incorporating lysozyme-loaded Ca-alginate microparticles into chitosan matrix. In these materials, the Ca-alginate microparticle carriers can fast release large amount of lysozyme at higher pH to compensate the decrease in enzyme activity, and decrease the release amount at lower pH when lysozyme presents a high activity. Degradation study revealed that the difference among the degradation profiles at various pH values is significantly decreased, indicating an anti-pH-interference effect of the composite films. The result well proved our designing ideas. In addition, the power law and Michaelis equations were used to figure out the inherent relationship between the drug release behavior and the degradation process. Finally, the fluorescence microscopy observation and MTT assay show that the degradation controllable materials have good biocompatibility, which allow adhesion and proliferation of the examined cells. (C) 2011 Elsevier Ltd. All rights reserved.