文献类型：期刊文献

英文题名：Circadian rhythm promotes the biomass and amylose hyperaccumulation by mixotrophic cultivation of marine microalga Platymonas helgolandica

作者：Shi, Qianwen[1];Chen, Cheng[1];He, Tingwei[3];Fan, Jianhua[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Dept Bioengn, Shanghai 200237, Peoples R China;[4]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832003, Peoples R China

年份：2022

卷号：15

期号：1

外文期刊名：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

收录：;EI(收录号：20222812334822);WOS:【SCI-EXPANDED(收录号:WOS:000825329800001)】；

基金：This work was sponsored by National Key Research and Development Project of China 2019YFA0906300 and 2020YFA0907304, Natural Science Foundation of Shandong Province ZR2019ZD17, Natural Science Foundation of Shanghai 21ZR1416400, Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Microalgae; Starch; Circadian rhythm; Mixotrophy; Amylose

摘要：Background Microalgal starch can be exploited for bioenergy, food, and bioplastics. Production of starch by green algae has been concerned for many years. Currently commonly used methods such as nutrient stress will affect cell growth, thereby inhibiting the production efficiency and quality of starch production. Simpler and more efficient control strategies need to be developed. Result We proposed a novel regulation method to promote the growth and starch accumulation by a newly isolated Chlorophyta Platymonas helgolandica. By adding exogenous glucose and controlling the appropriate circadian light and dark time, the highest dry weight accumulation 6.53 g L-1 (Light:Dark = 12:12) can be achieved, and the highest starch concentration could reach 3.88 g L-1 (Light:Dark = 6:18). The highest production rate was 0.40 g L-1 d(-1) after 9 days of production. And this method helps to improve the ability to produce amylose, with the highest accumulation of 39.79% DW amylose. We also discussed the possible mechanism of this phenomenon through revealing changes in the mRNA levels of key genes. Conclusion This study provides a new idea to regulate the production of amylose by green algae. For the first time, it is proposed to combine organic carbon source addition and circadian rhythm regulation to increase the starch production from marine green alga. A new starch-producing microalga has been isolated that can efficiently utilize organic matter and grow with or without photosynthesis.