文献类型：期刊文献

英文题名：A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain

作者：Shuai, Yuting[1,2];Ju, Yi[1,2];Li, Yuanhang[1,2];Ma, Dini[1,2,3];Jiang, Lan[1,2];Zhang, Jingyu[1,2];Tan, Gao-Yi[1,2];Liu, Xueting[1,2];Wang, Shenlin[1,2];Zhang, Lixin[1,2];Liu, Guang[1,2,3]

机构：[1]East China Univ Sci & Technol ECUST, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol ECUST, Sch Biotechnol, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Joint Int Res Lab Metab &Developmental Sci, Shanghai 200240, Peoples R China

年份：2023

卷号：8

期号：2

起止页码：213

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000948738500001)】；

基金：This work was supported by the National Natural Science Foundation of China (31900060) ; National Key Research and Development Program of China (2020YFA0907800, 2022YFC3400200, 2022YFA0912200) , Natural Science Foundation of Shanghai (20ZR1414500) , and Shanghai Pilot Program for Basic Research-Shanghai Jiao Tong University (21TQ1400204) .

语种：英文

外文关键词：Phosphorothioate; Sulfur binding domain; Nucleic acid detection

摘要：Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic acid detection techniques are challenged by trade-offs among speed, simplicity, precision and cost. Here, we described a novel method, designated SENSOR (Sulfur DNA mediated nucleic acid sensing platform), for rapid nucleic acid detection. SENSOR was developed from phosphorothioate (PT)-DNA and sulfur binding domain (SBD) which specifically binds double-stranded PT-modified DNA. SENSOR utilizes PT-DNA oligo and SBD as targeting module, which is linked with split luciferase reporter to generate luminescence signal within 10 min. We tested detection on synthesized nucleic acid and COVID-19 pseudovirus, achieving attomolar sensitivity combined with an amplification procedure. Single nucleotide polymorphisms (SNP) could also be discriminated. Indicating SENSOR a new promising nucleic acid detection technique.