文献类型：期刊文献

英文题名：Simultaneous quantification of fatty acids in serum with dual derivatization by liquid chromatography-tandem mass spectrometry

作者：Fu, Zhibo[1];Xu, Xuefeng[1];Lin, Chuhui[1];Yang, Haoyu[1];Zhao, Linghao[1];Zhou, Yuanyuan[1];Song, Yanbin[1];Zhang, Min[2];Zhang, Hongyang[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai, Peoples R China

年份：2023

卷号：46

期号：13

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20231914065806);WOS:【SCI-EXPANDED(收录号:WOS:000979948600001)】；

基金：ACKNOWLEDGMENTS This research was funded by the National Natural Science Foundation of China (Grant number 81973285).

语种：英文

外文关键词：dual derivatization; fatty acids; indole 3-carbohydrazide; indole 3-acetohydrazide; liquid chromatography-tandem mass spectrometry

摘要：Fatty acids have multitudinous biological functions and play a crucial role in many biological processes, but due to poor ionization efficiency and lack of appropriate internal standards, the comprehensive quantification of fatty acids by liquid chromatography-tandem mass spectrometry is still challenging. In this study, a new, accurate, and reliable method for quantifying 30 fatty acids in serum using dual derivatization was proposed. Indole-3-acetic acid hydrazide derivants of fatty acids were used as the internal standard and indole-3-carboxylic acid hydrazide derivants of them were used to quantify. The derivatization conditions were systematically optimized and the method validation results showed good linearity with R-2 > 0.9942, low detection limit (0.03-0.6 nM), precision (1.6%-9.8% for intra-day and 4.6%-14.1% for inter-day), recovery (88.2%-107.2% with relative standard deviation < 10.5%), matrix effect (88.3%-105.2% with the relative standard deviation < 9.9%) and stability (3.4%-13.8% for fatty acids derivants in 24 h at 4 degrees C and 4.2%-13.8% for three freeze-thaw cycles). Finally, this method was successfully applied to quantify fatty acids in serum samples of Alzheimer's patients. In contrast to the healthy control group, nine fatty acids showed a significant increase in the Alzheimer's disease group.