文献类型：期刊文献

中文题名：川陈皮素的体外抑癌活性及其机制研究

英文题名：In vitro anti-tumor activity of nobiletin against five human cancer cell lines and its mechanism

作者：苏明媛[1];牛江龙[1];李林[2];袁才根[2];卢艳花[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]联合利华中国研究所,联合利华研发中心上海,上海200335

年份：2011

卷号：33

期号：9

起止页码：1479

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：2009年度上海联合利华研究发展基金资助(09540715800);国家自然基金资助(30870253);国家重点实验室专项经费资助(2060204)

语种：中文

中文关键词：川陈皮素;人宫颈癌HeLa细胞;caspase-3;细胞凋亡

外文关键词：nobiletin； HeLa cells； caspase-3； apoptosis；

摘要：目的探讨川陈皮素对Caco-2、HeLa、HepG2、A375、SW1990 5种肿瘤细胞的增殖抑制作用及对HeLa细胞的作用机制。方法 0～200μmol/L浓度的川陈皮素作用于体外培养的肿瘤细胞,应用MTT法检测细胞存活率;流式细胞仪及Hochest33258染色检测细胞凋亡率及形态学变化;分光光度法检测凋亡蛋白酶caspase-3的变化。结果川陈皮素可显著抑制5种肿瘤细胞的生长,且对HeLa细胞的抑制效果最好。川陈皮素可促进HeLa细胞的凋亡,并可以增加其凋亡蛋白酶caspase-3的活性。结论川陈皮素能抑制5种肿瘤细胞尤其是HeLa细胞的生长。川陈皮素可诱导HeLa细胞发生凋亡;提高凋亡蛋白酶caspase-3的活性可能是其诱导细胞凋亡的作用机制之一。
AIM To investigate the anti-tumor effects of nobiletin on five human cancer cell lines Caco-2,HeLa,HepG2,A375,SW1990 and its anti-tumor mechanisms on HeLa cells.METHODS Five established human cancer cell lines in culture medium were treated with 0-200 μmoL/L nobiletin.The cell viability rate was measured by MTT assay.The cell apoptotic rate of HeLa cell was detected by flow cytometry and apoptotic morphology changes were studied by Hochest 33258 staining.The activity of caspase-3 was determined by spectrophotometrical analysis.RESULTS Nobiletin could inhibit the growth of five human cancer cell lines and has best inhibiting effect on HeLa cells.Nobiletin could increase the apoptotic rate of HeLa cells and also could increase the caspase-3 activity.CONCLUSION Nobiletin has apparent antitumor effects on these five human cancer cell lines,especially on HeLa cells,in vitro.Increasing the caspase-3 activity and promoting the apoptosis rate of HeLa cells may be one of its anti-tumor mechanisms.