文献类型：期刊文献

英文题名：Expanding horizons of CRISPR applications beyond genome editing

作者：Liang, Yu[1,2];Tong, Shengkun[1];Zhang, Jingyu[2,3];Tan, Gao-Yi[2];Zhang, Lixin[2,3];Lee, Sang Yup[4,5,6];Tong, Yaojun[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai 200237, Peoples R China;[4]Korea Adv Inst Sci & Technol KAIST, Dept Chem & Biomol Engn BK21 4, Metab & Biomol Engn Natl Res Lab, Daejeon 34141, South Korea;[5]Korea Adv Inst Sci & Technol, Bioproc Engn Res Ctr, Daejeon 34141, South Korea;[6]Korea Adv Inst Sci & Technol, Grad Sch Engn Biol, Daejeon 34141, South Korea

年份：2025

卷号：41

期号：10

起止页码：934

外文期刊名：TRENDS IN GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001594211600010)】；

语种：英文

摘要：Clustered regularly interspaced short palindromic repeats (CRISPR) technolo-gies have rapidly evolved beyond genome editing, transforming fields such as molecular diagnostics, biosensing, transcriptional regulation, molecular imag-ing, protein interaction mapping, and single-cell analysis. Emerging CRISPR-based diagnostics harness the collateral cleavage activity of CRISPR-associated (Cas) enzymes for rapid nucleic acid detection. Advanced biosensors extend CRISPR's capabilities to detect ions, metabolites, and proteins by integrating synthetic biology components. Catalytically inactive Cas proteins enable precise gene regulation and live-cell imaging of nucleic acids, whereas CRISPR-guided proximity labeling has revolutionized the mapping of biomolecular interactions. Recent single-cell CRISPR screens provide unprecedented resolution of cellular heterogeneity. Future research will focus on overcoming current limitations. The integration of CRISPR technologies with artificial intelligence (AI), spatial omics, and microfluidics is expected to further amplify their impact.