文献类型：期刊文献

中文题名：树脂吸附法从发酵液中提取红霉素的工艺研究

英文题名：Recovery of erythromycin from fermentation broth using membrane filtration and macroporous resin adsorption

作者：朱晟[1];朱家文[2];陈葵[2]

机构：[1]上海电力学院环境与化学工程学院,上海200090;[2]华东理工大学化学工程研究所,上海200237

年份：2017

卷号：45

期号：1

起止页码：22

中文期刊名：化学工程

外文期刊名：Chemical Engineering(China)

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

语种：中文

中文关键词：红霉素;发酵液;膜过滤;大孔树脂;吸附

外文关键词：erythromycin; fermentation broth; membrane filtration; macroporous resin; adsorption

摘要：针对传统红霉素生产工艺中存在的问题,提出了膜过滤、吸附、杂质洗涤、红霉素洗脱、洗脱液成盐及树脂再生等步骤组成的红霉素提取新工艺过程。以红霉素发酵液为原料,研究和优化了各单元操作的工艺条件和实际效果。采用孔径为50 nm的无机陶瓷膜对红霉素发酵液进行预处理,过程的平均脱色率为54.0%,平均脱蛋白率为67.5%,红霉素平均收率为92.5%。采用大孔树脂吸附技术对滤液中的有效成分进行浓缩、富集,过程收率为92.6%,结晶后所得红霉素硫氰酸盐的纯度相比原有工艺提高2.31%。筛选出体积分数50%丙酮和50%0.40mol/L NaOH溶液组成的混合溶液对使用后的树脂进行再生,再生后树脂的饱和吸附量不低于新鲜树脂饱和吸附量的80%。
To solve the problems in the traditional erythromycin（ EM） production process,a new process for recovery of EM from fermentation broth,including membrane filtration,macroporous resin adsorption,impurity washing,EM desorption, EM thiocyanate crystallization, and resin regeneration, was proposed. Using EM fermentation broth as starting material,the technological conditions and practical effects of each unit operation were studied and optimized. The ceramic membrane with the pore size of 50 nm was selected for the pretreatment of fermentation broth. The average decolorization ratio,the average deproteinization ratio and the average EM yield were 54. 0%,67. 5% and 92. 5%,respectively. The macroporous resin adsorption was used for the concentration of EM from filtrate. The purity of final product had an increase of 2. 31% compared with the original process with a yield of 92. 6%. The mixture of volume fraction 50% acetone and 50% 0. 40 mol / L NaOH solution was chosen to regenerate the exhausted resin,and the saturated adsorption capacity of the regenerated resin was no less than 80%of that of the fresh resin.