文献类型：期刊文献

英文题名：SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells

作者：Cui, Jing[1,2];Hu, Kai[1,2];Sun, Jia-Jia[1,2];Qu, Lu-Lu[3];Li, Da-Wei[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai Key Lab Funct Mat Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Jiangsu Normal Univ, Sch Chem & Chem Engn, Xuzhou 221116, Jiangsu, Peoples R China

年份：2016

卷号：85

起止页码：324

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20162002396308);WOS:【SCI-EXPANDED(收录号:WOS:000382410100043)】；

基金：This research was supported by the National Natural Science Foundation of China (21575041, 21505057), the Program of Shanghai Subject Chief Scientist (15XD1501200), and the Shanghai Municipal Natural Science Foundation (14ZR1410800).

语种：英文

外文关键词：SERS; Monitoring; Nitric oxide; Living cells

摘要：Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30 s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10(-7) M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems. (C) 2016 Elsevier B.V. All rights reserved.