文献类型：期刊文献

英文题名：Droplet-based dielectrophoresis device for on-chip nanomedicine fabrication and improved gene delivery efficiency

作者：Yang, Shih-Mo[1,2,3];Yao, Hong[1,2,4,5];Zhang, Dapeng[1,2];Li, Wen Jung[6];Kung, Hsiang-Fu[4,5];Chen, Shih-Chi[1,2]

机构：[1]Chinese Univ Hong Kong, Dept Mech & Automat Engn, Shatin, Hong Kong, Peoples R China;[2]Chinese Univ Hong Kong, Shun Hing Inst Adv Engn, Shatin, Hong Kong, Peoples R China;[3]E China Univ Sci & Technol, Sch Mech & Power Engn, Shanghai 200237, Peoples R China;[4]Chinese Univ Hong Kong, Sch Biomed Sci, Dept Surg, Shatin, Hong Kong, Peoples R China;[5]Chinese Univ Hong Kong, Stanley Ho Ctr Emerging Infect Dis, Shatin, Hong Kong, Peoples R China;[6]City Univ Hong Kong, Dept Mech & Biomed Engn, Kowloon Tong, Hong Kong, Peoples R China

年份：2015

卷号：19

期号：1

起止页码：235

外文期刊名：MICROFLUIDICS AND NANOFLUIDICS

收录：;EI(收录号：20150700523672);WOS:【SCI-EXPANDED(收录号:WOS:000356146300021)】；

基金：This research is supported by project #BME-p3-12 of the Shun Shing Institute of Advanced Engineering, The Chinese University of Hong Kong.

语种：英文

外文关键词：Droplet; Dielectrophoresis; Polymeric nanoparticle; Gene delivery; Particle separation; Lab-on-chip

摘要：In this article, we present the design, fabrication, and experimental verification of a droplet-based microfluidic device for effective on-chip fabrication and separation of polymer-based nanoparticles using dielectrophoresis (DEP) effect. The separated polyplexes nanoparticles were used in cells for improved gene transfection efficiency. By adjusting the flow rate of PEI600-CyD-FA (H-1) and DNA plasmids, polyplexes products can be mixed and self-assembled inside droplets within approximately a nanoliter volume. This procedure ensures synthesized particles to have a narrow size distribution. In addition, a new microchannel design was developed to automatically coalesce two moving aqueous droplets and to directly extract aqueous polyplex products from oil. Finally, the H-1-DNA polyplexes of similar to 116 nm diameter were separated via negative DEP force under 8 V (peak-peak) and 20 MHz conditions by passing three times through a non-uniform electric field. The biological findings demonstrated that the DEP-treated polyplexes still possessed the ability to enter HUVEC cells and that the gene transfection efficiency was raised to 15 %, as opposed to the control group's 4 % where the polyplexes had no DEP treatment. The quantitative comparison was done by counting the number of cells produced via positive EPFG expression. These hydrodynamic and electrodynamic techniques provide an integrated microfluidic platform for fabricating and screening nanoscale drugs.