文献类型：期刊文献

英文题名：Combining functional fucoidan with zein to enhance the adhesion and differentiation of myoblast cells

作者：Xiong, Wanli[1];Zhou, Yi[1];Chai, Miaomiao[1];Chen, Yawen[1];Dai, Wenjing[1];Wang, Lu[1];Ge, Chengxin[1];Tan, Wen-song[1];Zhou, Yan[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：221

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：;EI(收录号：20253318974658);WOS:【SCI-EXPANDED(收录号:WOS:001583182500007)】；

基金：The authors acknowledge financial support from State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.

语种：英文

外文关键词：Cultured meat; Co-assembly process; Myoblast adhesion; Myogenic differentiation; MAPK signaling pathway

摘要：Scaffolds play a pivotal role in cultured meat production. Plant protein-based scaffolds offer advantages such as superior nutritional value, sustainability, and low cost, but they often suffer from poor cell adhesion. Modifying plant protein scaffolds is an effective strategy to regulate myoblast attachment and differentiation. In this study, we developed an animal-free, edible scaffold through the co-assembly of zein and fucoidan. First, the study predicted the structure of alpha-zein and simulated its self-assembly into a stable 3D network via thermal evaporation in an alcohol solution using protein-protein interaction modeling. Further protein-molecule interaction analysis revealed the co-assembly process between zein and fucoidan. Fucoidan modification not only introduced nanotopography to the scaffold surface but also improved hydrophilicity and functionality, significantly enhancing cell adhesion-increasing attachment efficiency from 44 % to 81 %. Moreover, cells cultured on the composite scaffold exhibited superior proliferation and myogenic differentiation, with increased myotube length and fusion index. Mechanistic studies demonstrated that fucoidan enhances proliferation via the ERK pathway while synergistically regulating the phosphorylation of key proteins in the JNK and p38 MAPK pathways, thereby promoting C2C12 myogenic differentiation. These findings provide theoretical insights into fucoidan-mediated regulation of cellular activities and open new avenues for animal-free scaffolds in cultured meat and related applications.